Assay Detail
Binding
CHEMBL4481501
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Binding affinity to biotinylated full length human NLK expressed in Escherichia coli incubated for 1.5 hrs by fluorescent tracer dependent TR-FRET based competitive ligand binding displacement assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
SGC-GAK-1: A Chemical Probe for Cyclin G Associated Kinase (GAK).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 11 | 6.33 | 7.55 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4575895 | — | — | 1 | 7.55 |
| CHEMBL4585158 | — | — | 1 | 7.54 |
| CHEMBL530635 | — | — | 1 | 6.46 |
| CHEMBL311959 | — | — | 1 | 6.39 |
| CHEMBL4443342 | — | — | 1 | 6.28 |
| CHEMBL4556662 | — | — | 1 | 6.19 |
| CHEMBL4517424 | — | — | 1 | 6.18 |
| CHEMBL4586530 | — | — | 1 | 6.16 |
| CHEMBL535429 | — | — | 1 | 6.10 |
| CHEMBL4592501 | — | — | 1 | 5.58 |
| CHEMBL4586588 | — | — | 1 | 5.21 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4575895 | — | Kd | = | 28.0 | nM | 7.55 |
| CHEMBL4585158 | — | Kd | = | 29.0 | nM | 7.54 |
| CHEMBL530635 | — | Kd | = | 350.0 | nM | 6.46 |
| CHEMBL311959 | — | Kd | = | 410.0 | nM | 6.39 |
| CHEMBL4443342 | — | Kd | = | 520.0 | nM | 6.28 |
| CHEMBL4556662 | — | Kd | = | 650.0 | nM | 6.19 |
| CHEMBL4517424 | — | Kd | = | 660.0 | nM | 6.18 |
| CHEMBL4586530 | — | Kd | = | 700.0 | nM | 6.16 |
| CHEMBL535429 | — | Kd | = | 790.0 | nM | 6.10 |
| CHEMBL4592501 | — | Kd | = | 2600.0 | nM | 5.58 |
| CHEMBL4586588 | — | Kd | = | 6200.0 | nM | 5.21 |