Assay Detail
Binding
CHEMBL4603155
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of NAE-mediated neddylation in human MGC803 cells assessed as reduction in Nedd8-Ubcl2 adduct formation incubated for 24 hrs by Western blot analysis
2
Total Activities
1
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | MGC-803 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
NEDD8-activating enzyme E1 regulatory subunit (CHEMBL2016431) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Discovery of 1,2,4-triazine-based derivatives as novel neddylation inhibitors and anticancer activity studies against gastric cancer MGC-803 cells.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 1 | 5.45 | 5.45 |
| Inhibition | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4640167 | — | — | 2 | 5.45 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4640167 | — | EC50 | = | 3560.0 | nM | 5.45 |
| CHEMBL4640167 | — | Inhibition | - | % | - |