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Assay Detail

CHEMBL4605171

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to recombinant full-length GFP-fused CDK9/His-tagged cyclin T1 (unknown origin) expressed in HEK293T cells measured after 30 mins by microscale thermophoresis method
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK-293T
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

CDK9/cyclin T1 (CHEMBL2111389)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery and SARs of 5-Chloro-N4-phenyl-N2-(pyridin-2-yl)pyrimidine-2,4-diamine Derivatives as Oral Available and Dual CDK 6 and 9 Inhibitors with Potent Antitumor Activity.
Wang Y, Chen X, Yan Y, Zhu X, Liu M, Liu X.
J Med Chem (2020) 63 : 3327 -3347
PubMed 32129996 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 1 7.52 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4647703 1 7.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4647703 Kd = 30.0 nM 7.52