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Assay Detail

CHEMBL4610426

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of tracer 222 binding to recombinant human GST-tagged GAK (1 to 329 residues) expressed in insect cells incubated for 1 hr by Lanthascreen assay
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cyclin-G-associated kinase (CHEMBL4355)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationship study of the pyridine moiety of isothiazolo[4,3-b]pyridines as antiviral agents targeting cyclin G-associated kinase.
Martinez-Gualda B, Pu SY, Froeyen M, Herdewijn P, Einav S, De Jonghe S.
Bioorg Med Chem (2020) 28 : 115188 -115188
PubMed 31757682 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 6.80 7.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4646895 1 7.66
CHEMBL4642467 1 7.62
CHEMBL4172550 1 7.41
CHEMBL4645917 1 7.38
CHEMBL4642620 1 7.15
CHEMBL4636314 1 7.14
CHEMBL4643096 1 7.08
CHEMBL4641825 1 7.00
CHEMBL4632903 1 7.00
CHEMBL4639991 1 6.96
CHEMBL4638602 1 6.95
CHEMBL4632372 1 6.93
CHEMBL4633404 1 6.91
CHEMBL4642058 1 6.90
CHEMBL4640730 1 6.88
CHEMBL4635935 1 6.87
CHEMBL3425876 1 6.87
CHEMBL4640641 1 6.76
CHEMBL4648802 1 6.67
CHEMBL4638387 1 6.58
CHEMBL4648554 1 6.44
CHEMBL4635431 1 6.43
CHEMBL4637058 1 6.38
CHEMBL4634338 1 6.34
CHEMBL4636735 1 6.26
CHEMBL4642436 1 6.18
CHEMBL4639109 1 5.89
CHEMBL4636582 1 5.79
CHEMBL4648514 1 -
CHEMBL4644472 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4646895 IC50 = 22.0 nM 7.66
CHEMBL4642467 IC50 = 24.0 nM 7.62
CHEMBL4172550 IC50 = 39.4 nM 7.41
CHEMBL4645917 IC50 = 42.0 nM 7.38
CHEMBL4642620 IC50 = 71.0 nM 7.15
CHEMBL4636314 IC50 = 73.0 nM 7.14
CHEMBL4643096 IC50 = 83.0 nM 7.08
CHEMBL4641825 IC50 = 99.0 nM 7.00
CHEMBL4632903 IC50 = 101.0 nM 7.00
CHEMBL4639991 IC50 = 110.0 nM 6.96
CHEMBL4638602 IC50 = 113.0 nM 6.95
CHEMBL4632372 IC50 = 118.0 nM 6.93
CHEMBL4633404 IC50 = 124.0 nM 6.91
CHEMBL4642058 IC50 = 126.0 nM 6.90
CHEMBL4640730 IC50 = 132.0 nM 6.88
CHEMBL3425876 IC50 = 136.0 nM 6.87
CHEMBL4635935 IC50 = 136.0 nM 6.87
CHEMBL4640641 IC50 = 173.0 nM 6.76
CHEMBL4648802 IC50 = 214.0 nM 6.67
CHEMBL4638387 IC50 = 262.0 nM 6.58
CHEMBL4648554 IC50 = 364.0 nM 6.44
CHEMBL4635431 IC50 = 370.0 nM 6.43
CHEMBL4637058 IC50 = 422.0 nM 6.38
CHEMBL4634338 IC50 = 458.0 nM 6.34
CHEMBL4636735 IC50 = 545.0 nM 6.26
CHEMBL4642436 IC50 = 661.0 nM 6.18
CHEMBL4639109 IC50 = 1290.0 nM 5.89
CHEMBL4636582 IC50 = 1630.0 nM 5.79
CHEMBL4648514 IC50 > 370.0 nM -
CHEMBL4644472 IC50 > 370.0 nM -
CHEMBL4642223 IC50 > 10000.0 nM -
CHEMBL4638607 IC50 > 3330.0 nM -
CHEMBL4648166 IC50 > 3330.0 nM -