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Assay Detail

CHEMBL4612475

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of APC-labeled biotinylated-avidin from Euphorium-chelated recombinant human BRD4 bromodomain 1 expressed in Escherichia coli preincubated for 15 mins followed by APC-labeled biotinylated-avidin addition and measured after 1 hr under dark condition by TR-FRET assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel Pyrrolopyridone Bromodomain and Extra-Terminal Motif (BET) Inhibitors Effective in Endocrine-Resistant ER+ Breast Cancer with Acquired Resistance to Fulvestrant and Palbociclib.
Li Y, Zhao J, Gutgesell LM, Shen Z, Ratia K, Dye K, Dubrovskyi O, Zhao H, Huang F, Tonetti DA, Thatcher GRJ, Xiong R.
J Med Chem (2020) 63 : 7186 -7210
PubMed 32453591 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 6.98 8.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4648912 1 8.23
CHEMBL4640025 1 8.08
CHEMBL4647718 1 8.03
CHEMBL4633289 1 7.89
CHEMBL4632358 1 7.89
CHEMBL4644032 1 7.40
CHEMBL4640133 1 7.32
CHEMBL4646027 1 7.27
CHEMBL4638621 1 7.08
CHEMBL4637378 1 7.05
CHEMBL4646327 1 7.02
CHEMBL4644443 1 6.80
CHEMBL4649774 1 6.79
CHEMBL4632925 1 6.76
CHEMBL4647786 1 6.76
CHEMBL4635722 1 6.75
CHEMBL1957266 1 6.70
CHEMBL4639392 1 6.22
CHEMBL4636280 1 6.00
CHEMBL4634252 1 5.93
CHEMBL4642629 1 5.85
CHEMBL4644325 1 5.84

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4648912 IC50 = 5.9 nM 8.23
CHEMBL4640025 IC50 = 8.3 nM 8.08
CHEMBL4647718 IC50 = 9.4 nM 8.03
CHEMBL4633289 IC50 = 13.0 nM 7.89
CHEMBL4632358 IC50 = 12.9 nM 7.89
CHEMBL4644032 IC50 = 39.6 nM 7.40
CHEMBL4640133 IC50 = 48.0 nM 7.32
CHEMBL4646027 IC50 = 54.0 nM 7.27
CHEMBL4638621 IC50 = 83.0 nM 7.08
CHEMBL4637378 IC50 = 89.0 nM 7.05
CHEMBL4646327 IC50 = 96.0 nM 7.02
CHEMBL4644443 IC50 = 158.0 nM 6.80
CHEMBL4649774 IC50 = 161.0 nM 6.79
CHEMBL4632925 IC50 = 174.0 nM 6.76
CHEMBL4647786 IC50 = 175.0 nM 6.76
CHEMBL4635722 IC50 = 180.0 nM 6.75
CHEMBL1957266 IC50 = 199.0 nM 6.70
CHEMBL4639392 IC50 = 600.0 nM 6.22
CHEMBL4636280 IC50 = 1000.0 nM 6.00
CHEMBL4634252 IC50 = 1181.0 nM 5.93
CHEMBL4642629 IC50 = 1400.0 nM 5.85
CHEMBL4644325 IC50 = 1434.0 nM 5.84