Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4612964

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ERK5 in human HeLa cells assessed as reduction in EGF-induced ERK5 autophosphorylation pretreated for 1 hr followed by EGF stimulation and measured after 17 mins by 32p kinase assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 7 (CHEMBL5332)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and Structure-Activity Relationships of DCLK1 Kinase Inhibitors Based on a 5,11-Dihydro-6<i>H</i>-benzo[<i>e</i>]pyrimido[5,4-<i>b</i>][1,4]diazepin-6-one Scaffold.
Ferguson FM, Liu Y, Harshbarger W, Huang L, Wang J, Deng X, Capuzzi SJ, Muratov EN, Tropsha A, Muthuswamy S, Westover KD, Gray NS.
J Med Chem (2020) 63 : 7817 -7826
PubMed 32530623 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.39 7.18

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1673051 1 7.18
CHEMBL4636072 1 7.10
CHEMBL1673039 1 7.06
CHEMBL1673052 1 7.01
CHEMBL1673053 1 6.84
CHEMBL4648947 1 6.65
CHEMBL4636475 1 6.56
CHEMBL1673048 1 6.50
CHEMBL4643632 1 6.32
CHEMBL1673057 1 5.94
CHEMBL1672987 1 5.92
CHEMBL4634326 1 5.92
CHEMBL1673056 1 5.91
CHEMBL1673054 1 5.51
CHEMBL4639395 1 5.47
CHEMBL4638880 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1673051 IC50 = 66.0 nM 7.18
CHEMBL4636072 IC50 = 79.0 nM 7.10
CHEMBL1673039 IC50 = 87.0 nM 7.06
CHEMBL1673052 IC50 = 98.0 nM 7.01
CHEMBL1673053 IC50 = 146.0 nM 6.84
CHEMBL4648947 IC50 = 222.0 nM 6.65
CHEMBL4636475 IC50 = 276.0 nM 6.56
CHEMBL1673048 IC50 = 320.0 nM 6.50
CHEMBL4643632 IC50 = 482.0 nM 6.32
CHEMBL1673057 IC50 = 1138.0 nM 5.94
CHEMBL1672987 IC50 = 1200.0 nM 5.92
CHEMBL4634326 IC50 = 1200.0 nM 5.92
CHEMBL1673056 IC50 = 1240.0 nM 5.91
CHEMBL1673054 IC50 = 3100.0 nM 5.51
CHEMBL4639395 IC50 = 3350.0 nM 5.47
CHEMBL4638880 IC50 > 10000.0 nM -