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Assay Detail

CHEMBL4614818

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human HT-29 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Autocuration

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

Na<sup>+</sup>/K<sup>+</sup>-ATPase-Targeted Cytotoxicity of (+)-Digoxin and Several Semisynthetic Derivatives.
Ren Y, Ribas HT, Heath K, Wu S, Ren J, Shriwas P, Chen X, Johnson ME, Cheng X, Burdette JE, Kinghorn AD.
J Nat Prod (2020) 83 : 638 -648
PubMed 32096998 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.71 9.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL428647 PACLITAXEL 4.0 1 9.10
CHEMBL254219 DIGITOXIN 4.0 1 7.17
CHEMBL1751 DIGOXIN 4.0 1 6.55
CHEMBL1153 DIGOXIGENIN 1 5.44
CHEMBL4645702 1 5.29
CHEMBL4646176 1 -
CHEMBL4642134 1 -
CHEMBL4633690 1 -
CHEMBL4634645 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL428647 PACLITAXEL IC50 = 0.8 nM 9.10
CHEMBL254219 DIGITOXIN IC50 = 68.0 nM 7.17
CHEMBL1751 DIGOXIN IC50 = 280.0 nM 6.55
CHEMBL1153 DIGOXIGENIN IC50 = 3600.0 nM 5.44
CHEMBL4645702 IC50 = 5100.0 nM 5.29
CHEMBL4646176 IC50 > 10000.0 nM -
CHEMBL4642134 IC50 > 10000.0 nM -
CHEMBL4633690 IC50 > 10000.0 nM -
CHEMBL4634645 IC50 > 10000.0 nM -