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Assay Detail

CHEMBL4615734

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HDAC6 in mouse N2A cells assessed as increase in alpha tubulin acetylation
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type N2a
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 6 (CHEMBL2878)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Structural and in Vivo Characterization of Tubastatin A, a Widely Used Histone Deacetylase 6 Inhibitor.
Shen S, Svoboda M, Zhang G, Cavasin MA, Motlova L, McKinsey TA, Eubanks JH, Bařinka C, Kozikowski AP.
ACS Med Chem Lett (2020) 11 : 706 -712
PubMed 32435374 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 6.49 6.84

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2018302 TUBASTATIN A 1 6.84
CHEMBL4636622 1 6.60
CHEMBL3970950 1 6.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2018302 TUBASTATIN A EC50 = 145.0 nM 6.84
CHEMBL4636622 EC50 = 250.0 nM 6.60
CHEMBL3970950 EC50 = 932.0 nM 6.03