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Assay Detail

CHEMBL4618042

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of acetylcholinesterase (unknown origin) using acetylthiocholine as substrate preincubated for 30 mins followed by substrate addition and measured after 2 mins by Ellman's method
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL220)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Furofuranone Lignans from <i>Leucophyllum ambiguum</i>.
Rios MY, Ocampo-Acuña YD, Ramírez-Cisneros MÁ, Salazar-Rios ME.
J Nat Prod (2020) 83 : 1424 -1431
PubMed 32239935 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.93 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4647106 1 9.00
CHEMBL4634863 1 8.52
CHEMBL4633410 1 7.55
CHEMBL4633613 1 6.80
CHEMBL1980572 1 6.34
CHEMBL94 PHYSOSTIGMINE 3.0 1 6.27
CHEMBL454592 1 6.21
CHEMBL519926 1 6.04
CHEMBL4632461 1 5.65

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4647106 IC50 = 1.0 nM 9.00
CHEMBL4634863 IC50 = 3.0 nM 8.52
CHEMBL4633410 IC50 = 28.0 nM 7.55
CHEMBL4633613 IC50 = 158.0 nM 6.80
CHEMBL1980572 IC50 = 460.0 nM 6.34
CHEMBL94 PHYSOSTIGMINE IC50 = 538.0 nM 6.27
CHEMBL454592 IC50 = 616.0 nM 6.21
CHEMBL519926 IC50 = 903.0 nM 6.04
CHEMBL4632461 IC50 = 2229.0 nM 5.65