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Assay Detail

CHEMBL4619351

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Induction of ERalpha degradation in human MCF7 cells after 4 hrs by FITC/Hoechst staining based immunofluorescence imaging analysis
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MCF7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of GNE-149 as a Full Antagonist and Efficient Degrader of Estrogen Receptor alpha for ER+ Breast Cancer.
Liang J, Blake R, Chang J, Friedman LS, Goodacre S, Hartman S, Ingalla ER, Kiefer JR, Kleinheinz T, Labadie S, Li J, Lai KW, Liao J, Mody V, McLean N, Metcalfe C, Nannini M, Otwine D, Ran Y, Ray N, Roussel F, Sambrone A, Sampath D, Vinogradova M, Wai J, Wang T, Yeap K, Young A, Zbieg J, Zhang B, Zheng X, Zhong Y, Wang X.
ACS Med Chem Lett (2020) 11 : 1342 -1347
PubMed 32551022 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 9.73 10.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4447340 1 10.52
CHEMBL4649161 1 10.28
CHEMBL4635493 1 10.19
CHEMBL4646522 1 10.08
CHEMBL4644486 1 10.05
CHEMBL4648503 1 10.05
CHEMBL4644074 1 9.82
CHEMBL4641732 1 9.77
CHEMBL4640902 1 9.74
CHEMBL4528514 1 9.72
CHEMBL4633886 1 9.72
CHEMBL4645364 1 9.68
CHEMBL1358 FULVESTRANT 4.0 1 9.49
CHEMBL4644611 1 9.42
CHEMBL4641773 1 9.41
CHEMBL4638997 1 9.28
CHEMBL4634404 1 9.26
CHEMBL4640074 1 9.25
CHEMBL3581693 BRILANESTRANT 2.0 1 9.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4447340 IC50 = 0.03 nM 10.52
CHEMBL4649161 IC50 = 0.053 nM 10.28
CHEMBL4635493 IC50 = 0.065 nM 10.19
CHEMBL4646522 IC50 = 0.083 nM 10.08
CHEMBL4644486 IC50 = 0.09 nM 10.05
CHEMBL4648503 IC50 = 0.09 nM 10.05
CHEMBL4644074 IC50 = 0.15 nM 9.82
CHEMBL4641732 IC50 = 0.17 nM 9.77
CHEMBL4640902 IC50 = 0.18 nM 9.74
CHEMBL4528514 IC50 = 0.19 nM 9.72
CHEMBL4633886 IC50 = 0.19 nM 9.72
CHEMBL4645364 IC50 = 0.21 nM 9.68
CHEMBL1358 FULVESTRANT IC50 = 0.32 nM 9.49
CHEMBL4644611 IC50 = 0.38 nM 9.42
CHEMBL4641773 IC50 = 0.39 nM 9.41
CHEMBL4638997 IC50 = 0.52 nM 9.28
CHEMBL4634404 IC50 = 0.55 nM 9.26
CHEMBL4640074 IC50 = 0.56 nM 9.25
CHEMBL3581693 BRILANESTRANT IC50 = 0.85 nM 9.07