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Assay Detail

CHEMBL4620359

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human TDO expressed in Escherichia coli BL21 using L-Trp as substrate incubated for 30 mins by enzymatic assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tryptophan 2,3-dioxygenase (CHEMBL2140)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of novel tryptanthrin derivatives as dual inhibitors of indoleamine 2,3-dioxygenase 1 and tryptophan 2,3-dioxygenase.
Li Y, Zhang S, Wang R, Cui M, Liu W, Yang Q, Kuang C.
Bioorg Med Chem Lett (2020) 30 : 127159 -127159
PubMed 32247733 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.21 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4645973 1 7.52
CHEMBL4643029 1 7.22
CHEMBL4647345 1 6.75
CHEMBL4646483 1 6.52
CHEMBL4648181 1 6.43
CHEMBL4633682 1 6.38
CHEMBL4646657 1 6.35
CHEMBL4645897 1 6.00
CHEMBL4644380 1 5.95
CHEMBL4635512 1 5.92
CHEMBL4643729 1 5.54
CHEMBL4644195 1 5.22
CHEMBL1812545 1 4.94
CHEMBL3545369 EPACADOSTAT 3.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4645973 IC50 = 30.0 nM 7.52
CHEMBL4643029 IC50 = 60.0 nM 7.22
CHEMBL4647345 IC50 = 180.0 nM 6.75
CHEMBL4646483 IC50 = 300.0 nM 6.52
CHEMBL4648181 IC50 = 370.0 nM 6.43
CHEMBL4633682 IC50 = 420.0 nM 6.38
CHEMBL4646657 IC50 = 450.0 nM 6.35
CHEMBL4645897 IC50 = 990.0 nM 6.00
CHEMBL4644380 IC50 = 1130.0 nM 5.95
CHEMBL4635512 IC50 = 1210.0 nM 5.92
CHEMBL4643729 IC50 = 2870.0 nM 5.54
CHEMBL4644195 IC50 = 6020.0 nM 5.22
CHEMBL1812545 IC50 = 11580.0 nM 4.94
CHEMBL3545369 EPACADOSTAT IC50 - -