Assay Detail
Binding
CHEMBL4621698
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human C-terminal Ig-Fc-tagged PD1 (Leu25 to Gln167 residues) expressed in HEK293 cells/human C-terminal epitope-His-tagged PDL1 (Phe19 to Arg238 residues) expressed in HEK293 cells protein-protein interaction preincubated for 15 mins with PDL1 followed by PD1 addition and measured after 15 mins by APC-labeled anti-His antibody/Eu-labeled anti-human IgG based HTRF assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
Programmed cell death protein 1/Programmed cell death 1 ligand 1 (CHEMBL4523993) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
Discovery of Novel Resorcinol Dibenzyl Ethers Targeting the Programmed Cell Death-1/Programmed Cell Death-Ligand 1 Interaction as Potential Anticancer Agents.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 7.55 | 8.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4646293 | — | — | 1 | 8.10 |
| CHEMBL4648161 | — | — | 1 | 8.00 |
| CHEMBL4639202 | — | — | 1 | 7.90 |
| CHEMBL4638536 | — | — | 1 | 6.93 |
| CHEMBL4099869 | — | — | 1 | 6.84 |
| CHEMBL4643215 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4646293 | — | IC50 | = | 7.9 | nM | 8.10 |
| CHEMBL4648161 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL4639202 | — | IC50 | = | 12.5 | nM | 7.90 |
| CHEMBL4638536 | — | IC50 | = | 118.5 | nM | 6.93 |
| CHEMBL4099869 | — | IC50 | = | 146.0 | nM | 6.84 |
| CHEMBL4643215 | — | IC50 | > | 100.0 | nM | - |