Assay Detail
Binding
CHEMBL4621974
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli using L-arginine as substrate preincubated for 15 mins followed by NADPH addition and measured after 20 mins by OPA/NAC reagent based HPLC-fluorimetric analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Antiglioma Activity of Aryl and Amido-Aryl Acetamidine Derivatives Targeting iNOS: Synthesis and Biological Evaluation.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 6.90 | 7.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4633346 | — | — | 1 | 7.96 |
| CHEMBL107251 | 1400W | — | 1 | 7.10 |
| CHEMBL3589087 | — | — | 1 | 6.37 |
| CHEMBL4635568 | — | — | 1 | 6.17 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4633346 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL107251 | 1400W | IC50 | = | 80.0 | nM | 7.10 |
| CHEMBL3589087 | — | IC50 | = | 428.0 | nM | 6.37 |
| CHEMBL4635568 | — | IC50 | = | 681.0 | nM | 6.17 |