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Assay Detail

CHEMBL4622222

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 2-((1E,3E,5E)-5-(3,3-Dimethyl-1-(6-((4-(((3-methyl-5-(1H-pyrazolo[3,4-b]pyridin-4-yl)pyridin-2-yl)oxy)methyl)bicyclo[2.2.2]octan-1-yl)amino)-6-oxohexyl)-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-5-sulfo-3H-indol-1-ium trifluoroacetate from biotinylated human TLR8 extracellular domain (27 to 827 residues) expressed in Drosophila S2 cells after 30 mins by europium-labeled streptavidin based TR-FRET assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Toll-like receptor 8 (CHEMBL5805)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Target-Based Identification and Optimization of 5-Indazol-5-yl Pyridones as Toll-like Receptor 7 and 8 Antagonists Using a Biochemical TLR8 Antagonist Competition Assay.
Knoepfel T, Nimsgern P, Jacquier S, Bourrel M, Vangrevelinghe E, Glatthar R, Behnke D, Alper PB, Michellys PY, Deane J, Junt T, Zipfel G, Limonta S, Hawtin S, Andre C, Boulay T, Loetscher P, Faller M, Blank J, Feifel R, Betschart C.
J Med Chem (2020) 63 : 8276 -8295
PubMed 32786235 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 5.93 7.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4634860 1 7.17
CHEMBL4642652 1 7.16
CHEMBL4647012 1 6.85
CHEMBL4637821 1 6.66
CHEMBL4632846 1 6.57
CHEMBL4647559 1 6.55
CHEMBL4644098 1 6.50
CHEMBL4634366 1 6.40
CHEMBL4649727 1 6.22
CHEMBL4633872 1 6.10
CHEMBL4647201 1 5.89
CHEMBL4633436 1 5.85
CHEMBL4635744 1 5.85
CHEMBL4646609 1 5.80
CHEMBL4643502 1 5.77
CHEMBL4632520 1 5.72
CHEMBL4640935 1 5.70
CHEMBL4635717 1 5.68
CHEMBL4646719 1 5.62
CHEMBL4648996 1 5.57
CHEMBL4633423 1 5.50
CHEMBL4641604 1 5.43
CHEMBL4635547 1 5.38
CHEMBL4639145 1 5.12
CHEMBL4642538 1 4.72
CHEMBL4647602 1 4.31
CHEMBL4641633 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4634860 IC50 = 68.0 nM 7.17
CHEMBL4642652 IC50 = 69.0 nM 7.16
CHEMBL4647012 IC50 = 140.0 nM 6.85
CHEMBL4637821 IC50 = 220.0 nM 6.66
CHEMBL4632846 IC50 = 270.0 nM 6.57
CHEMBL4647559 IC50 = 280.0 nM 6.55
CHEMBL4644098 IC50 = 320.0 nM 6.50
CHEMBL4634366 IC50 = 400.0 nM 6.40
CHEMBL4649727 IC50 = 600.0 nM 6.22
CHEMBL4633872 IC50 = 800.0 nM 6.10
CHEMBL4647201 IC50 = 1300.0 nM 5.89
CHEMBL4633436 IC50 = 1400.0 nM 5.85
CHEMBL4635744 IC50 = 1400.0 nM 5.85
CHEMBL4646609 IC50 = 1600.0 nM 5.80
CHEMBL4643502 IC50 = 1700.0 nM 5.77
CHEMBL4632520 IC50 = 1900.0 nM 5.72
CHEMBL4640935 IC50 = 2000.0 nM 5.70
CHEMBL4635717 IC50 = 2100.0 nM 5.68
CHEMBL4646719 IC50 = 2400.0 nM 5.62
CHEMBL4648996 IC50 = 2700.0 nM 5.57
CHEMBL4633423 IC50 = 3200.0 nM 5.50
CHEMBL4641604 IC50 = 3700.0 nM 5.43
CHEMBL4635547 IC50 = 4200.0 nM 5.38
CHEMBL4639145 IC50 = 7600.0 nM 5.12
CHEMBL4642538 IC50 = 19000.0 nM 4.72
CHEMBL4647602 IC50 = 49000.0 nM 4.31
CHEMBL4641633 IC50 < 20.0 nM -