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Assay Detail

CHEMBL4627014

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human 5HT2A/mGlu2 receptor expressed in HEK293 cells co-expressing Gqo5 assessed as inhibition of glutamate-induced intracellular Ca2+ mobilization incubated for 1 hr by Fluo-4 dye based fluorescence assay 5HT2A_HUMAN/GRM2_HUMAN P28223/Q14416
27
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Publication

Design, Synthesis, and Pharmacological Characterization of Heterobivalent Ligands for the Putative 5-HT<sub>2A</sub>/mGlu<sub>2</sub> Receptor Complex.
Poulie CBM, Liu N, Jensen AA, Bunch L.
J Med Chem (2020) 63 : 9928 -9949
PubMed 32815361 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 6.41 7.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL489624 2 7.64
CHEMBL4638006 2 7.62
CHEMBL4636980 2 7.54
CHEMBL4645193 2 6.18
CHEMBL4635960 2 6.16
CHEMBL4638687 2 6.05
CHEMBL4639821 2 6.00
CHEMBL4641860 2 5.93
CHEMBL4635373 2 5.72
CHEMBL4642761 2 5.28
CHEMBL4632373 1 -
CHEMBL4643961 1 -
CHEMBL4635729 1 -
CHEMBL4645305 1 -
CHEMBL4646555 1 -
CHEMBL4646835 1 -
CHEMBL2206440 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL489624 IC50 = 22.91 nM 7.64
CHEMBL489624 IC50 = 23.0 nM 7.64
CHEMBL4638006 IC50 = 24.0 nM 7.62
CHEMBL4638006 IC50 = 23.99 nM 7.62
CHEMBL4636980 IC50 = 29.0 nM 7.54
CHEMBL4636980 IC50 = 28.84 nM 7.54
CHEMBL4645193 IC50 = 660.69 nM 6.18
CHEMBL4645193 IC50 = 660.0 nM 6.18
CHEMBL4635960 IC50 = 691.83 nM 6.16
CHEMBL4635960 IC50 = 700.0 nM 6.16
CHEMBL4638687 IC50 = 890.0 nM 6.05
CHEMBL4638687 IC50 = 891.25 nM 6.05
CHEMBL4639821 IC50 = 1000.0 nM 6.00
CHEMBL4639821 IC50 = 990.0 nM 6.00
CHEMBL4641860 IC50 = 1174.9 nM 5.93
CHEMBL4641860 IC50 = 1200.0 nM 5.92
CHEMBL4635373 IC50 = 1900.0 nM 5.72
CHEMBL4635373 IC50 = 1905.46 nM 5.72
CHEMBL4642761 IC50 = 5300.0 nM 5.28
CHEMBL4642761 IC50 = 5248.07 nM 5.28
CHEMBL2206440 IC50 - -
CHEMBL4646835 IC50 - -
CHEMBL4632373 IC50 - -
CHEMBL4643961 IC50 - -
CHEMBL4635729 IC50 - -
CHEMBL4645305 IC50 - -
CHEMBL4646555 IC50 - -