Assay Detail
Binding
CHEMBL4627506
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [3H]17-beta estradiol from GST-fused human recombinant ERbeta ligand binding domain expressed in Escherichia coli BL21alpha cells incubated for 1 hr by liquid scintillation counting method
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Bisphenol AF: Halogen bonding effect is a major driving force for the dual ERα-agonist and ERβ-antagonist activities.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 7 | 7.38 | 9.17 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL135 | ESTRADIOL | 4.0 | 1 | 9.17 |
| CHEMBL4642117 | — | — | 1 | 8.13 |
| CHEMBL1900054 | — | — | 1 | 7.83 |
| CHEMBL196585 | — | — | 1 | 7.81 |
| CHEMBL4646249 | — | — | 1 | 7.01 |
| CHEMBL418971 | BISPHENOL A | — | 1 | 6.06 |
| CHEMBL2392656 | — | — | 1 | 5.62 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL135 | ESTRADIOL | IC50 | = | 0.67 | nM | 9.17 |
| CHEMBL4642117 | — | IC50 | = | 7.39 | nM | 8.13 |
| CHEMBL1900054 | — | IC50 | = | 14.7 | nM | 7.83 |
| CHEMBL196585 | — | IC50 | = | 15.4 | nM | 7.81 |
| CHEMBL4646249 | — | IC50 | = | 96.8 | nM | 7.01 |
| CHEMBL418971 | BISPHENOL A | IC50 | = | 870.0 | nM | 6.06 |
| CHEMBL2392656 | — | IC50 | = | 2400.0 | nM | 5.62 |