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Assay Detail

CHEMBL4628267

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse recombinant C-terminal FLAG-tagged SMS2 expressed in mammalian expression system using C14-phosphatidylcholineD72 and C17-ceramide pre-incubated for 60 mins followed by incubation with substrate for 30 mins measured by RapidFire/MS assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of 1,8-naphthyridin-2-one derivative as a potent and selective sphingomyelin synthase 2 inhibitor.
Yukawa T, Nakahata T, Okamoto R, Ishichi Y, Miyamoto Y, Nishimura S, Oikawa T, Kubo K, Adachi R, Satomi Y, Nakakariya M, Amano N, Kamaura M, Matsunaga N.
Bioorg Med Chem (2020) 28 : 115376 -115376
PubMed 32088125 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.16 8.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4645607 1 8.66
CHEMBL4641888 1 8.52
CHEMBL4646354 1 8.11
CHEMBL4644293 1 7.85
CHEMBL4638337 1 7.72
CHEMBL4646540 1 7.50
CHEMBL4639746 1 7.29
CHEMBL4634697 1 6.92
CHEMBL4638097 1 6.72
CHEMBL4634669 1 6.62
CHEMBL4637599 1 6.58
CHEMBL4635147 1 6.47
CHEMBL4649105 1 6.23
CHEMBL4644982 1 6.18
CHEMBL4162182 1 5.96
CHEMBL4647686 1 -
CHEMBL4633986 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4645607 IC50 = 2.2 nM 8.66
CHEMBL4641888 IC50 = 3.0 nM 8.52
CHEMBL4646354 IC50 = 7.7 nM 8.11
CHEMBL4644293 IC50 = 14.0 nM 7.85
CHEMBL4638337 IC50 = 19.0 nM 7.72
CHEMBL4646540 IC50 = 32.0 nM 7.50
CHEMBL4639746 IC50 = 51.0 nM 7.29
CHEMBL4634697 IC50 = 120.0 nM 6.92
CHEMBL4638097 IC50 = 190.0 nM 6.72
CHEMBL4634669 IC50 = 240.0 nM 6.62
CHEMBL4637599 IC50 = 260.0 nM 6.58
CHEMBL4635147 IC50 = 340.0 nM 6.47
CHEMBL4649105 IC50 = 590.0 nM 6.23
CHEMBL4644982 IC50 = 660.0 nM 6.18
CHEMBL4162182 IC50 = 1100.0 nM 5.96
CHEMBL4647686 IC50 - -
CHEMBL4633986 IC50 - -