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Assay Detail

CHEMBL4628612

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR in human KB cells assessed as reduction in EGF-stimulated EGFR phosphorylation preincubated for 1 hr followed by EGF stimulation and measured after 6 mins by ELISA
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type KB
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of a novel class EGFR/HER2 dual inhibitors containing tricyclic oxazine fused quinazolines scaffold.
Sun M, Jia J, Sun H, Wang F.
Bioorg Med Chem Lett (2020) 30 : 127045 -127045
PubMed 32139324 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 6.68 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2441563 1 7.92
CHEMBL553 ERLOTINIB 4.0 1 7.72
CHEMBL939 GEFITINIB 4.0 1 7.60
CHEMBL4643111 1 7.35
CHEMBL554 LAPATINIB 4.0 1 7.21
CHEMBL4636996 1 7.10
CHEMBL4642158 1 6.94
CHEMBL4634499 1 6.92
CHEMBL4635100 1 6.92
CHEMBL2441567 1 6.90
CHEMBL4648271 1 6.79
CHEMBL4648924 1 6.75
CHEMBL4634817 1 6.35
CHEMBL4634127 1 6.25
CHEMBL4643903 1 6.24
CHEMBL4638194 1 6.07
CHEMBL4639556 1 5.31
CHEMBL4641942 1 5.31
CHEMBL4646638 1 5.23
CHEMBL4632500 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2441563 IC50 = 12.0 nM 7.92
CHEMBL553 ERLOTINIB IC50 = 19.0 nM 7.72
CHEMBL939 GEFITINIB IC50 = 25.0 nM 7.60
CHEMBL4643111 IC50 = 45.0 nM 7.35
CHEMBL554 LAPATINIB IC50 = 62.0 nM 7.21
CHEMBL4636996 IC50 = 80.0 nM 7.10
CHEMBL4642158 IC50 = 116.0 nM 6.94
CHEMBL4634499 IC50 = 120.0 nM 6.92
CHEMBL4635100 IC50 = 121.0 nM 6.92
CHEMBL2441567 IC50 = 126.0 nM 6.90
CHEMBL4648271 IC50 = 163.0 nM 6.79
CHEMBL4648924 IC50 = 179.0 nM 6.75
CHEMBL4634817 IC50 = 446.0 nM 6.35
CHEMBL4634127 IC50 = 569.0 nM 6.25
CHEMBL4643903 IC50 = 578.0 nM 6.24
CHEMBL4638194 IC50 = 844.0 nM 6.07
CHEMBL4639556 IC50 = 4870.0 nM 5.31
CHEMBL4641942 IC50 = 4870.0 nM 5.31
CHEMBL4646638 IC50 = 5830.0 nM 5.23
CHEMBL4632500 IC50 > 10000.0 nM -