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Assay Detail

CHEMBL4628613

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR (unknown origin) using FAM-labeled peptide as substrate preincubated for 10 mins followed by substrate addition measured after 40 mins in presence of ATP by caliper mobility shift assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of a novel class EGFR/HER2 dual inhibitors containing tricyclic oxazine fused quinazolines scaffold.
Sun M, Jia J, Sun H, Wang F.
Bioorg Med Chem Lett (2020) 30 : 127045 -127045
PubMed 32139324 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 8.33 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL939 GEFITINIB 4.0 1 9.00
CHEMBL553 ERLOTINIB 4.0 1 9.00
CHEMBL4643111 1 8.22
CHEMBL2441563 1 8.15
CHEMBL4635100 1 8.15
CHEMBL4648271 1 8.10
CHEMBL554 LAPATINIB 4.0 1 7.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL939 GEFITINIB IC50 = 1.0 nM 9.00
CHEMBL553 ERLOTINIB IC50 = 1.0 nM 9.00
CHEMBL4643111 IC50 = 6.0 nM 8.22
CHEMBL2441563 IC50 = 7.0 nM 8.15
CHEMBL4635100 IC50 = 7.0 nM 8.15
CHEMBL4648271 IC50 = 8.0 nM 8.10
CHEMBL554 LAPATINIB IC50 = 22.0 nM 7.66