Assay Detail
Binding
CHEMBL4629572
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at recombinant human pFA-CMV fused PPARgamma expressed in HEK293T cells transfected with pFR-luciferase plasmid and pRL-SV40 plasmid incubated for 14 to 16 hrs by dual glo luciferase reporter gene assay
8
Total Activities
8
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK-293T |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Peroxisome proliferator-activated receptor gamma (CHEMBL235) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
l-Thyroxin and the Nonclassical Thyroid Hormone TETRAC Are Potent Activators of PPARγ.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 7 | 5.77 | 7.00 |
| Activity | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL549748 | TETRAC | -1.0 | 1 | 7.00 |
| CHEMBL4637025 | — | — | 1 | 6.52 |
| CHEMBL41632 | TIRATRICOL | 3.0 | 1 | 6.43 |
| CHEMBL1743304 | REVERSE TRIIODOTHYRONINE | — | 1 | 5.42 |
| CHEMBL1544 | LIOTHYRONINE | 4.0 | 1 | 5.24 |
| CHEMBL1624 | LEVOTHYROXINE | 4.0 | 1 | 4.92 |
| CHEMBL4638870 | — | — | 1 | 4.89 |
| CHEMBL595 | PIOGLITAZONE | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL549748 | TETRAC | EC50 | = | 100.0 | nM | 7.00 |
| CHEMBL4637025 | — | EC50 | = | 300.0 | nM | 6.52 |
| CHEMBL41632 | TIRATRICOL | EC50 | = | 370.0 | nM | 6.43 |
| CHEMBL1743304 | REVERSE TRIIODOTHYRONINE | EC50 | = | 3800.0 | nM | 5.42 |
| CHEMBL1544 | LIOTHYRONINE | EC50 | = | 5800.0 | nM | 5.24 |
| CHEMBL1624 | LEVOTHYROXINE | EC50 | = | 12000.0 | nM | 4.92 |
| CHEMBL4638870 | — | EC50 | = | 12900.0 | nM | 4.89 |
| CHEMBL595 | PIOGLITAZONE | Activity | - | — | - |