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Assay Detail

CHEMBL4630441

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at CCR4 in human CCRF-CEM cells assessed as inhibition of CCL22-mediated chemotaxis preincubated for 30 mins followed by CCL22 addition and measured after 60 mins in presence of 100% human serum by CellTiter-Glo assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CCRF-CEM
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

C-C chemokine receptor type 4 (CHEMBL2414)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel Piperidinyl-Azetidines as Potent and Selective CCR4 Antagonists Elicit Antitumor Response as a Single Agent and in Combination with Checkpoint Inhibitors.
Robles O, Jackson JJ, Marshall L, Talay O, Chian D, Cutler G, Diokno R, Hu DX, Jacobson S, Karbarz E, Kassner PD, Ketcham JM, McKinnell J, Meleza C, Reilly MK, Riegler E, Shunatona HP, Wadsworth A, Younai A, Brockstedt DG, Wustrow DJ, Zibinsky M.
J Med Chem (2020) 63 : 8584 -8607
PubMed 32667798 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 6.67 7.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4642563 1 7.44
CHEMBL4641127 1 7.40
CHEMBL4637143 1 7.30
CHEMBL4645325 1 7.23
CHEMBL4634054 1 7.13
CHEMBL4639600 1 7.01
CHEMBL4641873 1 6.96
CHEMBL4636749 1 6.94
CHEMBL4641595 1 6.88
CHEMBL4647188 1 6.87
CHEMBL4640439 1 6.61
CHEMBL4647117 1 6.59
CHEMBL4643158 1 6.43
CHEMBL4637695 1 6.42
CHEMBL4639418 1 6.39
CHEMBL4633133 1 6.35
CHEMBL4633494 1 6.28
CHEMBL4638846 1 6.25
CHEMBL4633920 1 6.24
CHEMBL4643609 1 6.14
CHEMBL4645037 1 6.11
CHEMBL4640909 1 5.82
CHEMBL4643839 1 -
CHEMBL4644412 1 -
CHEMBL4636965 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4642563 IC50 = 36.0 nM 7.44
CHEMBL4641127 IC50 = 40.0 nM 7.40
CHEMBL4637143 IC50 = 50.0 nM 7.30
CHEMBL4645325 IC50 = 59.0 nM 7.23
CHEMBL4634054 IC50 = 74.0 nM 7.13
CHEMBL4639600 IC50 = 97.0 nM 7.01
CHEMBL4641873 IC50 = 109.0 nM 6.96
CHEMBL4636749 IC50 = 115.0 nM 6.94
CHEMBL4641595 IC50 = 133.0 nM 6.88
CHEMBL4647188 IC50 = 136.0 nM 6.87
CHEMBL4640439 IC50 = 247.0 nM 6.61
CHEMBL4647117 IC50 = 259.0 nM 6.59
CHEMBL4643158 IC50 = 370.0 nM 6.43
CHEMBL4637695 IC50 = 379.0 nM 6.42
CHEMBL4639418 IC50 = 409.0 nM 6.39
CHEMBL4633133 IC50 = 446.0 nM 6.35
CHEMBL4633494 IC50 = 523.0 nM 6.28
CHEMBL4638846 IC50 = 559.0 nM 6.25
CHEMBL4633920 IC50 = 575.0 nM 6.24
CHEMBL4643609 IC50 = 717.0 nM 6.14
CHEMBL4645037 IC50 = 775.0 nM 6.11
CHEMBL4640909 IC50 = 1500.0 nM 5.82
CHEMBL4643839 IC50 - -
CHEMBL4644412 IC50 - -
CHEMBL4636965 IC50 - -