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Assay Detail

CHEMBL4650004

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Binding
Alphascreen assay. Binding to BRD4A (domain start/stop: N44-E168) by alphascreen assay
8
Total Activities
5
Compounds Tested
1
Activity Types
3
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Category Alphascreen assay
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

EUbOPEN bromodomain assay panel
SGC
(2021)
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.97 7.59

Assay Parameters

Parameter Type Value Units Text
Protein Sequence Protein Sequence - MHHHHHHSSGVDLGTENLYFQSMNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEE
Refseq Start/Stop Refseq Start/Stop - N44-E168
Target UniProt Accession Target UniProt Accession - O60885

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2132936 1 7.59
CHEMBL3133807 3 7.08
CHEMBL3774655 1 5.61
CHEMBL3622373 2 5.15
CHEMBL3769507 1 4.95

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2132936 IC50 = 25.4 nM 7.59
CHEMBL3133807 IC50 = 83.0 nM 7.08
CHEMBL3133807 IC50 = 310.9 nM 6.51
CHEMBL3133807 IC50 = 468.4 nM 6.33
CHEMBL3774655 IC50 = 2456.1 nM 5.61
CHEMBL3622373 IC50 = 7121.2 nM 5.15
CHEMBL3769507 IC50 = 11190.0 nM 4.95
CHEMBL3622373 IC50 = 27790.0 nM 4.56