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Assay Detail

CHEMBL4667901

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human SPPL2a expressed in HEK293 cells cotransfected with Gal4 luciferase reporter plasmid and VP16-TNFalpha(1-76)-NTF incubated for 24 hrs by luciferase reporter gene assay based luminescence assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Signal peptide peptidase-like 2A (CHEMBL4105833)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Orally Active Hydroxyethylamine Based SPPL2a Inhibitors.
Velcicky J,Mathison CJN,Nikulin V,Pflieger D,Epple R,Azimioara M,Cow C,Michellys PY,Rigollier P,Beisner DR,Bodendorf U,Guerini D,Liu B,Wen B,Zaharevitz S,Brandl T
ACS Med Chem Lett (2019) 10 : 887 -892
PubMed 31223443 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.76 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4762492 1 8.15
CHEMBL4759048 1 8.05
CHEMBL4753654 1 7.89
CHEMBL4792187 1 7.82
CHEMBL4759943 1 7.60
CHEMBL4740837 1 7.06
CHEMBL4762370 1 6.70
CHEMBL4747630 1 6.46
CHEMBL4789381 1 6.21
CHEMBL4785324 1 6.08
CHEMBL4743609 1 6.07
CHEMBL4753079 1 6.05
CHEMBL4798232 1 6.04
CHEMBL4741014 1 5.82
CHEMBL4762488 1 5.37

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4762492 IC50 = 7.0 nM 8.15
CHEMBL4759048 IC50 = 9.0 nM 8.05
CHEMBL4753654 IC50 = 13.0 nM 7.89
CHEMBL4792187 IC50 = 15.0 nM 7.82
CHEMBL4759943 IC50 = 25.0 nM 7.60
CHEMBL4740837 IC50 = 88.0 nM 7.06
CHEMBL4762370 IC50 = 200.0 nM 6.70
CHEMBL4747630 IC50 = 350.0 nM 6.46
CHEMBL4789381 IC50 = 620.0 nM 6.21
CHEMBL4785324 IC50 = 840.0 nM 6.08
CHEMBL4743609 IC50 = 860.0 nM 6.07
CHEMBL4753079 IC50 = 900.0 nM 6.05
CHEMBL4798232 IC50 = 910.0 nM 6.04
CHEMBL4741014 IC50 = 1500.0 nM 5.82
CHEMBL4762488 IC50 = 4300.0 nM 5.37