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Assay Detail

CHEMBL4668253

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human unphosphorylated BTK using FITC-Ahx-TSELKKVVALYDYMPMNAND-NH2 as substrate incubated for 60 mins in presence of ATP at Km concentration
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design of Potent and Selective Covalent Inhibitors of Bruton's Tyrosine Kinase Targeting an Inactive Conformation.
Pulz R,Angst D,Dawson J,Gessier F,Gutmann S,Hersperger R,Hinniger A,Janser P,Koch G,Revesz L,Vulpetti A,Waelchli R,Zimmerlin A,Cenni B
ACS Med Chem Lett (2019) 10 : 1467 -1472
PubMed 31620235 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 8.19 10.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4762397 1 10.85
CHEMBL4749522 1 9.22
CHEMBL4593663 1 9.05
CHEMBL4739958 1 9.00
CHEMBL4795673 1 8.92
CHEMBL4746262 1 8.85
CHEMBL4740933 1 8.30
CHEMBL1873475 IBRUTINIB 4.0 1 8.30
CHEMBL4789435 1 8.22
CHEMBL4566818 1 7.89
CHEMBL4793026 1 7.58
CHEMBL4788086 1 6.72
CHEMBL4744271 1 6.41
CHEMBL4752522 1 5.41

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4762397 IC50 = 0.014 nM 10.85
CHEMBL4749522 IC50 = 0.6 nM 9.22
CHEMBL4593663 IC50 = 0.9 nM 9.05
CHEMBL4739958 IC50 = 1.0 nM 9.00
CHEMBL4795673 IC50 = 1.2 nM 8.92
CHEMBL4746262 IC50 = 1.4 nM 8.85
CHEMBL4740933 IC50 = 5.0 nM 8.30
CHEMBL1873475 IBRUTINIB IC50 = 5.0 nM 8.30
CHEMBL4789435 IC50 = 6.0 nM 8.22
CHEMBL4566818 IC50 = 13.0 nM 7.89
CHEMBL4793026 IC50 = 26.0 nM 7.58
CHEMBL4788086 IC50 = 190.0 nM 6.72
CHEMBL4744271 IC50 = 390.0 nM 6.41
CHEMBL4752522 IC50 = 3900.0 nM 5.41