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Assay Detail

CHEMBL4668599

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of human BSEP expressed in HEK293 cell membrane vesicles assessed as reduction in 3H-TCA uptake incubated for 5 mins by radiodetection method
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bile salt export pump (CHEMBL6020)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Drug Induced Liver Injury (DILI). Mechanisms and Medicinal Chemistry Avoidance/Mitigation Strategies.
Norman BH
J Med Chem (2020) 63 : 11397 -11419
PubMed 32511920 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.42 6.58

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL595 PIOGLITAZONE 4.0 1 6.58
CHEMBL160 CYCLOSPORINE 4.0 1 6.40
CHEMBL163 RITONAVIR 4.0 1 5.66
CHEMBL114 SAQUINAVIR 4.0 1 5.54
CHEMBL157101 KETOCONAZOLE 4.0 1 5.47
CHEMBL121 ROSIGLITAZONE 4.0 1 5.42
CHEMBL472 GLYBURIDE 4.0 1 5.28
CHEMBL408 TROGLITAZONE 4.0 1 5.23
CHEMBL554 LAPATINIB 4.0 1 5.19
CHEMBL623 NEFAZODONE 4.0 1 5.16
CHEMBL1513 IRBESARTAN 4.0 1 5.14
CHEMBL1484 NICARDIPINE 4.0 1 5.10
CHEMBL772 RESERPINE 4.0 1 5.08
CHEMBL191 LOSARTAN 4.0 1 5.07
CHEMBL54349 ALPIDEM 4.0 1 5.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL595 PIOGLITAZONE IC50 = 260.0 nM 6.58
CHEMBL160 CYCLOSPORINE IC50 = 400.0 nM 6.40
CHEMBL163 RITONAVIR IC50 = 2200.0 nM 5.66
CHEMBL114 SAQUINAVIR IC50 = 2900.0 nM 5.54
CHEMBL157101 KETOCONAZOLE IC50 = 3400.0 nM 5.47
CHEMBL121 ROSIGLITAZONE IC50 = 3800.0 nM 5.42
CHEMBL472 GLYBURIDE IC50 = 5300.0 nM 5.28
CHEMBL408 TROGLITAZONE IC50 = 5900.0 nM 5.23
CHEMBL554 LAPATINIB IC50 = 6500.0 nM 5.19
CHEMBL623 NEFAZODONE IC50 = 7000.0 nM 5.16
CHEMBL1513 IRBESARTAN IC50 = 7300.0 nM 5.14
CHEMBL1484 NICARDIPINE IC50 = 7900.0 nM 5.10
CHEMBL772 RESERPINE IC50 = 8400.0 nM 5.08
CHEMBL191 LOSARTAN IC50 = 8500.0 nM 5.07
CHEMBL54349 ALPIDEM IC50 = 9200.0 nM 5.04