Assay Detail
Binding
CHEMBL4668636
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of FITC-labeled Bak BH3 peptide binding to His6-tagged GST-fused Bcl-xL (unknown origin) expressed in Escherichia coli BL21 preincubated for 10 mins followed by Bak BH3 peptide addition and measured after 30 mins by fluorescence polarization-based competitive binding assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Fragment Linking Strategies for Structure-Based Drug Design.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 7.10 | 7.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL383229 | — | — | 1 | 7.10 |
| CHEMBL4747123 | — | — | 1 | - |
| CHEMBL4749218 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL383229 | — | IC50 | = | 78.8 | nM | 7.10 |
| CHEMBL4747123 | — | IC50 | > | 100000.0 | nM | - |
| CHEMBL4749218 | — | IC50 | > | 100000.0 | nM | - |