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Assay Detail

CHEMBL4670679

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at DOP (unknown origin) expressed in SH-SY5Y cell membranes co-expressing beta-arrestin2-RGFP protein assessed as induction of beta-arrestin2 activation incubated for 15 mins by BRET assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SH-SY5Y
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Delta-type opioid receptor (CHEMBL236)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Biased Agonism at Nociceptin/Orphanin FQ Receptors: A Structure Activity Study on N/OFQ(1-13)-NH.
Pacifico S,Ferrari F,Albanese V,Marzola E,Neto JA,Ruzza C,Calò G,Preti D,Guerrini R
J Med Chem (2020) 63 : 10782 -10795
PubMed 32901477 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 7.19 7.83

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL273820 1 7.83
CHEMBL4749747 1 7.65
CHEMBL8234 ENKEPHALIN 1 7.37
CHEMBL4747671 1 6.88
CHEMBL4789975 1 6.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL273820 EC50 = 14.79 nM 7.83
CHEMBL4749747 EC50 = 22.39 nM 7.65
CHEMBL8234 ENKEPHALIN EC50 = 42.66 nM 7.37
CHEMBL4747671 EC50 = 131.83 nM 6.88
CHEMBL4789975 EC50 = 616.6 nM 6.21