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Assay Detail

CHEMBL4670927

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibiton of EGFR L858R/T790M mutant (unknown origin) expressed in human NCI-H1975 cells assessed as reduction in EGFR induced cell viability after 96 hrs by CellTiter-Glo assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type NCI-H1975
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Targeting Her2-insYVMA with Covalent Inhibitors-A Focused Compound Screening and Structure-Based Design Approach.
Lategahn J,Hardick J,Grabe T,Niggenaber J,Jeyakumar K,Keul M,Tumbrink HL,Becker C,Hodson L,Kirschner T,Klövekorn P,Ketzer J,Baumann M,Terheyden S,Unger A,Weisner J,Müller MP,van Otterlo WAL,Bauer S,Rauh D
J Med Chem (2020) 63 : 11725 -11755
PubMed 32931277 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.07 7.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3353410 OSIMERTINIB 4.0 1 7.66
CHEMBL1173655 AFATINIB 4.0 1 6.15
CHEMBL4787483 1 6.13
CHEMBL4757145 1 6.09
CHEMBL4795165 1 5.57
CHEMBL554 LAPATINIB 4.0 1 4.83
CHEMBL180022 NERATINIB 4.0 1 -
CHEMBL4756368 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3353410 OSIMERTINIB IC50 = 22.0 nM 7.66
CHEMBL1173655 AFATINIB IC50 = 714.0 nM 6.15
CHEMBL4787483 IC50 = 739.0 nM 6.13
CHEMBL4757145 IC50 = 807.0 nM 6.09
CHEMBL4795165 IC50 = 2664.0 nM 5.57
CHEMBL554 LAPATINIB IC50 = 14781.0 nM 4.83
CHEMBL180022 NERATINIB IC50 < 14.0 nM -
CHEMBL4756368 IC50 - -