Assay Detail
Binding
CHEMBL4675252
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human CYP1A1 expressed in human HEK293 cells using 7-ethoxyresorufin as substrate preincubated for 30 mins followed by substrate additon and measured after 60 mins by fluorescence based assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Furanoflavones pongapin and lanceolatin B blocks the cell cycle and induce senescence in CYP1A1-overexpressing breast cancer cells.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 6.42 | 7.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL577397 | PONGAPIN | — | 1 | 7.70 |
| CHEMBL224157 | LANCEOLATIN B | — | 1 | 7.70 |
| CHEMBL208484 | KARANJIN | — | 1 | 7.52 |
| CHEMBL4763663 | — | — | 1 | 6.62 |
| CHEMBL573847 | OVALITENONE | — | 1 | 6.32 |
| CHEMBL4740121 | — | — | 1 | 6.31 |
| CHEMBL458131 | PONGAMOL | — | 1 | 5.36 |
| CHEMBL583961 | PONGAGLABRONE | — | 1 | 5.19 |
| CHEMBL4793151 | — | — | 1 | 5.07 |
| CHEMBL283196 | ALPHA-NAPHTHOFLAVONE | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL577397 | PONGAPIN | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL224157 | LANCEOLATIN B | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL208484 | KARANJIN | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL4763663 | — | IC50 | = | 240.0 | nM | 6.62 |
| CHEMBL573847 | OVALITENONE | IC50 | = | 480.0 | nM | 6.32 |
| CHEMBL4740121 | — | IC50 | = | 490.0 | nM | 6.31 |
| CHEMBL458131 | PONGAMOL | IC50 | = | 4400.0 | nM | 5.36 |
| CHEMBL583961 | PONGAGLABRONE | IC50 | = | 6400.0 | nM | 5.19 |
| CHEMBL4793151 | — | IC50 | = | 8600.0 | nM | 5.07 |
| CHEMBL283196 | ALPHA-NAPHTHOFLAVONE | IC50 | > | 10000.0 | nM | - |