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Assay Detail

CHEMBL4676183

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at rat GnRH receptor expressed in CHO-K1 cells assessed as inhibition of LHRH-induced response preincubated for 20 mins followed by LHRH addition and measured after 60 mins by TR-FRET assay
12
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Gonadotropin-releasing hormone receptor (CHEMBL3066)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Discovery and Characterization of BAY 1214784, an Orally Available Spiroindoline Derivative Acting as a Potent and Selective Antagonist of the Human Gonadotropin-Releasing Hormone Receptor as Proven in a First-In-Human Study in Postmenopausal Women.
Panknin O,Wagenfeld A,Bone W,Bender E,Nowak-Reppel K,Fernández-Montalván AE,Nubbemeyer R,Bäurle S,Ring S,Schmees N,Prien O,Schäfer M,Friedrich C,Zollner TM,Steinmeyer A,Mueller T,Langer G
J Med Chem (2020) 63 : 11854 -11881
PubMed 32960053 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.97 7.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4740373 2 7.57
CHEMBL4749790 2 7.54
CHEMBL4756920 2 7.40
CHEMBL4798298 1 7.14
CHEMBL4740709 4 6.14
CHEMBL4787218 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4740373 IC50 = 27.0 nM 7.57
CHEMBL4740373 IC50 = 27.0 nM 7.57
CHEMBL4749790 IC50 = 29.0 nM 7.54
CHEMBL4756920 IC50 = 40.0 nM 7.40
CHEMBL4756920 IC50 = 40.0 nM 7.40
CHEMBL4798298 IC50 = 72.0 nM 7.14
CHEMBL4740709 IC50 = 730.0 nM 6.14
CHEMBL4740709 IC50 = 726.0 nM 6.14
CHEMBL4740709 IC50 = 1419.0 nM 5.85
CHEMBL4749790 IC50 >= 20.0 10^3nM -
CHEMBL4740709 IC50 >= 20.0 10^3nM -
CHEMBL4787218 IC50 - -