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Assay Detail

CHEMBL4678926

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CDK9 in human MV4-11 cells assessed as induction of caspase-3/7 activation measured after 6 hrs followed by Caspase-glo reagent based assay
30
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MV4-11
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cyclin-dependent kinase 9 (CHEMBL3116)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of AZD4573, a Potent and Selective Inhibitor of CDK9 That Enables Short Duration of Target Engagement for the Treatment of Hematological Malignancies.
Barlaam B,Casella R,Cidado J,Cook C,De Savi C,Dishington A,Donald CS,Drew L,Ferguson AD,Ferguson D,Glossop S,Grebe T,Gu C,Hande S,Hawkins J,Hird AW,Holmes J,Horstick J,Jiang Y,Lamb ML,McGuire TM,Moore JE,O'Connell N,Pike A,Pike KG,Proia T,Roberts B,San Martin M,Sarkar U,Shao W,Stead D,Sumner N,Thakur K,Vasbinder MM,Varnes JG,Wang J,Wang L,Wu D,Wu L,Yang B,Yao T
J Med Chem (2020) 63 : 15564 -15590
PubMed 33306391 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 30 6.76 7.86

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4462530 ZEMIRCICLIB 2.0 1 7.86
CHEMBL4784117 1 7.82
CHEMBL4752748 1 7.82
CHEMBL4754081 1 7.54
CHEMBL4740254 1 7.42
CHEMBL4785242 1 7.41
CHEMBL4749175 1 7.27
CHEMBL4759074 2 7.17
CHEMBL4753076 1 7.13
CHEMBL4753256 2 7.11
CHEMBL4741063 1 7.00
CHEMBL4754399 1 6.89
CHEMBL4784363 1 6.85
CHEMBL4759053 1 6.82
CHEMBL4751230 1 6.80
CHEMBL4786995 1 6.75
CHEMBL3673434 1 6.66
CHEMBL4796500 1 6.58
CHEMBL4788016 1 6.48
CHEMBL4798156 1 6.38
CHEMBL4780912 1 6.36
CHEMBL4758821 1 6.25
CHEMBL4760601 1 6.18
CHEMBL4750648 1 6.17
CHEMBL4756050 1 6.16
CHEMBL4764361 1 6.04
CHEMBL4789009 1 5.92
CHEMBL4787309 1 5.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4462530 ZEMIRCICLIB EC50 = 13.7 nM 7.86
CHEMBL4784117 EC50 = 15.0 nM 7.82
CHEMBL4752748 EC50 = 15.0 nM 7.82
CHEMBL4754081 EC50 = 29.0 nM 7.54
CHEMBL4740254 EC50 = 38.0 nM 7.42
CHEMBL4785242 EC50 = 39.0 nM 7.41
CHEMBL4749175 EC50 = 54.0 nM 7.27
CHEMBL4759074 EC50 = 68.0 nM 7.17
CHEMBL4753076 EC50 = 74.0 nM 7.13
CHEMBL4753256 EC50 = 77.0 nM 7.11
CHEMBL4753256 EC50 = 92.0 nM 7.04
CHEMBL4741063 EC50 = 100.0 nM 7.00
CHEMBL4754399 EC50 = 130.0 nM 6.89
CHEMBL4784363 EC50 = 140.0 nM 6.85
CHEMBL4759053 EC50 = 150.0 nM 6.82
CHEMBL4751230 EC50 = 160.0 nM 6.80
CHEMBL4786995 EC50 = 180.0 nM 6.75
CHEMBL3673434 EC50 = 220.0 nM 6.66
CHEMBL4796500 EC50 = 260.0 nM 6.58
CHEMBL4788016 EC50 = 330.0 nM 6.48
CHEMBL4798156 EC50 = 420.0 nM 6.38
CHEMBL4780912 EC50 = 440.0 nM 6.36
CHEMBL4758821 EC50 = 560.0 nM 6.25
CHEMBL4760601 EC50 = 660.0 nM 6.18
CHEMBL4750648 EC50 = 670.0 nM 6.17
CHEMBL4756050 EC50 = 700.0 nM 6.16
CHEMBL4764361 EC50 = 910.0 nM 6.04
CHEMBL4789009 EC50 = 1200.0 nM 5.92
CHEMBL4787309 EC50 = 1900.0 nM 5.72
CHEMBL4759074 EC50 = 4800.0 nM 5.32