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Assay Detail

CHEMBL4679296

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]alpha-bungarotoxin from alpha7nAChR in rat hippocampus membranes preincubated for 5 mins followed by [125I]alpha-bungarotoxin addition and measured after overnight incubation by liquid scintillation beta counter analysis
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Hippocampus
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor subunit alpha-7 (CHEMBL4980)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Modifications at C(5) of 2-(2-Pyrrolidinyl)-Substituted 1,4-Benzodioxane Elicit Potent α4β2 Nicotinic Acetylcholine Receptor Partial Agonism with High Selectivity over the α3β4 Subtype.
Bavo F,Pallavicini M,Gotti C,Appiani R,Moretti M,Colombo SF,Pucci S,Viani P,Budriesi R,Renzi M,Fucile S,Bolchi C
J Med Chem (2020) 63 : 15668 -15692
PubMed 33325696 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 31 4.88 6.63

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3 NICOTINE 4.0 1 6.63
CHEMBL1917231 1 6.37
CHEMBL4755768 1 5.68
CHEMBL4785648 1 5.58
CHEMBL4762584 1 5.09
CHEMBL4792797 1 4.98
CHEMBL4798883 1 4.98
CHEMBL4744398 1 4.87
CHEMBL4758050 1 4.72
CHEMBL1917232 1 4.70
CHEMBL1186866 1 4.68
CHEMBL4778976 1 4.57
CHEMBL4783660 1 4.50
CHEMBL1917229 1 4.44
CHEMBL4792229 1 4.38
CHEMBL4741357 1 4.35
CHEMBL4747289 1 4.34
CHEMBL4784011 1 4.30
CHEMBL1917230 1 4.29
CHEMBL4095303 1 4.05
CHEMBL4740421 1 -
CHEMBL4788079 1 -
CHEMBL4799765 1 -
CHEMBL4760797 1 -
CHEMBL4776494 1 -
CHEMBL4762415 1 -
CHEMBL4783337 1 -
CHEMBL4790023 1 -
CHEMBL4782049 1 -
CHEMBL4743341 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3 NICOTINE Ki = 234.0 nM 6.63
CHEMBL1917231 Ki = 430.0 nM 6.37
CHEMBL4755768 Ki = 2100.0 nM 5.68
CHEMBL4785648 Ki = 2600.0 nM 5.58
CHEMBL4762584 Ki = 8100.0 nM 5.09
CHEMBL4792797 Ki = 10400.0 nM 4.98
CHEMBL4798883 Ki = 10500.0 nM 4.98
CHEMBL4744398 Ki = 13500.0 nM 4.87
CHEMBL4758050 Ki = 19100.0 nM 4.72
CHEMBL1917232 Ki = 20000.0 nM 4.70
CHEMBL1186866 Ki = 21000.0 nM 4.68
CHEMBL4778976 Ki = 26800.0 nM 4.57
CHEMBL4783660 Ki = 31500.0 nM 4.50
CHEMBL1917229 Ki = 36000.0 nM 4.44
CHEMBL4792229 Ki = 42200.0 nM 4.38
CHEMBL4741357 Ki = 44500.0 nM 4.35
CHEMBL4747289 Ki = 45500.0 nM 4.34
CHEMBL4784011 Ki = 50000.0 nM 4.30
CHEMBL1917230 Ki = 51000.0 nM 4.29
CHEMBL4095303 Ki = 89000.0 nM 4.05
CHEMBL4740421 Ki - -
CHEMBL4788079 Ki - -
CHEMBL4799765 Ki > 10000.0 nM -
CHEMBL4760797 Ki > 10000.0 nM -
CHEMBL4776494 Ki - -
CHEMBL4762415 Ki > 10000.0 nM -
CHEMBL4783337 Ki = 109000.0 nM -
CHEMBL4790023 Ki - -
CHEMBL4782049 Ki - -
CHEMBL4743341 Ki - -
CHEMBL4759608 Ki - -