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Assay Detail

CHEMBL4685312

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of recombinant human N-terminal GST-tagged TYK2 cytoplasmic domain (833 to 1187 residues) expressed in baculovirus expression system using TK-substrate-biotin preincubated for 5 mins followed by substrate addition and measured after 30 mins by HTRF assay
40
Total Activities
40
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Non-receptor tyrosine-protein kinase TYK2 (CHEMBL3553)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and pharmacological evaluation of 4- or 6-phenyl-pyrimidine derivatives as novel and selective Janus kinase 3 inhibitors.
Shu L,Chen C,Huan X,Huang H,Wang M,Zhang J,Yan Y,Liu J,Zhang T,Zhang D
Eur J Med Chem (2020) 191 : 112148 -112148
PubMed 32097841 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 40 5.84 7.45

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL221959 TOFACITINIB 4.0 1 7.45
CHEMBL4755671 1 5.73
CHEMBL4741588 1 5.64
CHEMBL4758891 1 5.61
CHEMBL4744519 1 5.60
CHEMBL4743357 1 5.58
CHEMBL4746272 1 5.55
CHEMBL4754557 1 5.54
CHEMBL4740169 1 -
CHEMBL4756089 1 -
CHEMBL4782611 1 -
CHEMBL4750184 1 -
CHEMBL1078166 1 -
CHEMBL4740889 1 -
CHEMBL4793519 1 -
CHEMBL4761545 1 -
CHEMBL4750831 1 -
CHEMBL4777645 1 -
CHEMBL4778584 1 -
CHEMBL4747498 1 -
CHEMBL4777465 1 -
CHEMBL1077575 1 -
CHEMBL4746760 1 -
CHEMBL4780644 1 -
CHEMBL4795109 1 -
CHEMBL4796206 1 -
CHEMBL4758931 1 -
CHEMBL4756648 1 -
CHEMBL4754061 1 -
CHEMBL4762354 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL221959 TOFACITINIB IC50 = 35.5 nM 7.45
CHEMBL4755671 IC50 = 1872.0 nM 5.73
CHEMBL4741588 IC50 = 2264.0 nM 5.64
CHEMBL4758891 IC50 = 2439.0 nM 5.61
CHEMBL4744519 IC50 = 2496.0 nM 5.60
CHEMBL4743357 IC50 = 2653.0 nM 5.58
CHEMBL4746272 IC50 = 2783.0 nM 5.55
CHEMBL4754557 IC50 = 2871.0 nM 5.54
CHEMBL4793519 IC50 - -
CHEMBL4740191 IC50 - -
CHEMBL4761545 IC50 - -
CHEMBL4750831 IC50 - -
CHEMBL4777645 IC50 - -
CHEMBL4778584 IC50 - -
CHEMBL4756089 IC50 > 3000.0 nM -
CHEMBL4749364 IC50 - -
CHEMBL4780984 IC50 > 3000.0 nM -
CHEMBL4739907 IC50 - -
CHEMBL4753620 IC50 - -
CHEMBL4763424 IC50 > 3000.0 nM -
CHEMBL4778980 IC50 > 3000.0 nM -
CHEMBL4778274 IC50 - -
CHEMBL4750671 IC50 - -
CHEMBL4789894 IC50 - -
CHEMBL4740889 IC50 - -
CHEMBL1078166 IC50 > 3000.0 nM -
CHEMBL4750184 IC50 > 3000.0 nM -
CHEMBL4782611 IC50 > 3000.0 nM -
CHEMBL4740169 IC50 - -
CHEMBL4747498 IC50 - -
CHEMBL4777465 IC50 - -
CHEMBL1077575 IC50 > 3000.0 nM -
CHEMBL4746760 IC50 - -
CHEMBL4780644 IC50 - -
CHEMBL4795109 IC50 - -
CHEMBL4796206 IC50 - -
CHEMBL4758931 IC50 - -
CHEMBL4756648 IC50 - -
CHEMBL4754061 IC50 - -
CHEMBL4762354 IC50 - -