Assay Detail
Binding
CHEMBL4685845
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human N-terminal GST-tagged/C-terminal His-tagged HDAC4 (627 to 1084 residues) expressed in baculovirus expression system using Boc-Lys(TFA)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Synthesis and biological evaluation of acridine-based histone deacetylase inhibitors as multitarget agents against Alzheimer's disease.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 13 | 5.06 | 5.92 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4761847 | — | — | 1 | 5.92 |
| CHEMBL4762873 | — | — | 1 | 5.65 |
| CHEMBL4757035 | — | — | 1 | 5.52 |
| CHEMBL4248676 | — | — | 1 | 5.15 |
| CHEMBL4796066 | — | — | 1 | 5.11 |
| CHEMBL4751082 | — | — | 1 | 4.83 |
| CHEMBL4753795 | — | — | 1 | 4.80 |
| CHEMBL4757768 | — | — | 1 | 4.58 |
| CHEMBL4753828 | — | — | 1 | 4.53 |
| CHEMBL98 | VORINOSTAT | 4.0 | 1 | 4.48 |
| CHEMBL4750838 | — | — | 1 | - |
| CHEMBL4799871 | — | — | 1 | - |
| CHEMBL4752558 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4761847 | — | IC50 | = | 1210.0 | nM | 5.92 |
| CHEMBL4762873 | — | IC50 | = | 2240.0 | nM | 5.65 |
| CHEMBL4757035 | — | IC50 | = | 3040.0 | nM | 5.52 |
| CHEMBL4248676 | — | IC50 | = | 7060.0 | nM | 5.15 |
| CHEMBL4796066 | — | IC50 | = | 7850.0 | nM | 5.11 |
| CHEMBL4751082 | — | IC50 | = | 14710.0 | nM | 4.83 |
| CHEMBL4753795 | — | IC50 | = | 15730.0 | nM | 4.80 |
| CHEMBL4757768 | — | IC50 | = | 26630.0 | nM | 4.58 |
| CHEMBL4753828 | — | IC50 | = | 29380.0 | nM | 4.53 |
| CHEMBL98 | VORINOSTAT | IC50 | = | 33040.0 | nM | 4.48 |
| CHEMBL4750838 | — | IC50 | > | 40000.0 | nM | - |
| CHEMBL4799871 | — | IC50 | > | 40000.0 | nM | - |
| CHEMBL4752558 | — | IC50 | > | 40000.0 | nM | - |