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Assay Detail

CHEMBL4685966

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of fluorescent-labelled E2 from recombinant human GST-tagged ERalpha by LanthaScreen TR-FRET assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of bivalent triarylalkene- and cyclofenil-derived dual estrogen receptor antagonists and downregulators.
Knox A,Kalchschmid C,Schuster D,Gaggia F,Manzl C,Baecker D,Gust R
Eur J Med Chem (2020) 192 : 112191 -112191
PubMed 32169784 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 8.51 9.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL135 ESTRADIOL 4.0 1 9.48
CHEMBL4761289 1 9.38
CHEMBL4758245 1 8.89
CHEMBL4790840 1 8.78
CHEMBL279301 1 8.65
CHEMBL4790191 1 8.63
CHEMBL1358 FULVESTRANT 4.0 1 8.54
CHEMBL4755319 1 8.45
CHEMBL195515 1 8.27
CHEMBL4784145 1 8.19
CHEMBL4793264 1 7.87
CHEMBL4794674 1 7.82
CHEMBL4791340 1 7.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL135 ESTRADIOL IC50 = 0.33 nM 9.48
CHEMBL4761289 IC50 = 0.42 nM 9.38
CHEMBL4758245 IC50 = 1.28 nM 8.89
CHEMBL4790840 IC50 = 1.67 nM 8.78
CHEMBL279301 IC50 = 2.24 nM 8.65
CHEMBL4790191 IC50 = 2.35 nM 8.63
CHEMBL1358 FULVESTRANT IC50 = 2.85 nM 8.54
CHEMBL4755319 IC50 = 3.52 nM 8.45
CHEMBL195515 IC50 = 5.35 nM 8.27
CHEMBL4784145 IC50 = 6.43 nM 8.19
CHEMBL4793264 IC50 = 13.6 nM 7.87
CHEMBL4794674 IC50 = 15.1 nM 7.82
CHEMBL4791340 IC50 = 18.9 nM 7.72