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Assay Detail

CHEMBL4689737

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at recombinant GST-tagged FXR LBD (unknown origin) assessed as inhibition of GW4064-induced Fluorecein-SRC2-2 coactivator peptide recruitment by LanthaScreen TR-FRET co-regulator assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bile acid receptor (CHEMBL2047)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Orally Active and Nonsteroidal Farnesoid X Receptor (FXR) Antagonist with Propensity for Accumulation and Responsiveness in Ileum.
Teno N,Iguchi Y,Oda K,Yamashita Y,Masuda A,Fujimori K,Une M,Gohda K
ACS Med Chem Lett (2021) 12 : 420 -425
PubMed 33738070 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 7.40 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4552760 1 8.15
CHEMBL4797745 1 8.11
CHEMBL4783205 1 7.48
CHEMBL4453417 1 7.46
CHEMBL4785930 1 7.20
CHEMBL4783777 1 7.19
CHEMBL4778665 1 6.85
CHEMBL4749439 1 6.74

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4552760 IC50 = 7.0 nM 8.15
CHEMBL4797745 IC50 = 7.8 nM 8.11
CHEMBL4783205 IC50 = 32.9 nM 7.48
CHEMBL4453417 IC50 = 35.0 nM 7.46
CHEMBL4785930 IC50 = 62.6 nM 7.20
CHEMBL4783777 IC50 = 64.5 nM 7.19
CHEMBL4778665 IC50 = 140.0 nM 6.85
CHEMBL4749439 IC50 = 183.1 nM 6.74