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Assay Detail

CHEMBL4700097

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human HaCaT cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HaCaT
Confidence 1 — Organism
Curated By Autocuration

Target

HaCaT (CHEMBL614392)
Type CELL-LINE
Organism Homo sapiens

Publication

Allocolchicinoids bearing a Michael acceptor fragment for possible irreversible binding of tubulin
Sazanova, Ekaterina S., Gracheva, Iuliia A., Allegro, Diane, Barbier, Pascale, Combes, Sebastien, Svirshchevskaya, Elena V., Fedorov, Alexey Yu
RSC Med Chem (2020) 11 : 696 -706
PubMed 33479669 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 8.01 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4797732 1 9.22
CHEMBL4743585 1 9.22
CHEMBL4798577 1 8.52
CHEMBL4761555 1 8.52
CHEMBL4744305 1 8.52
CHEMBL107 COLCHICINE 4.0 1 8.52
CHEMBL4789567 1 7.80
CHEMBL4763029 1 7.80
CHEMBL4786968 1 7.80
CHEMBL4794634 1 7.80
CHEMBL4741408 1 7.80
CHEMBL4759719 1 7.10
CHEMBL4740897 1 7.10
CHEMBL4759452 1 6.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4797732 IC50 = 0.6 nM 9.22
CHEMBL4743585 IC50 = 0.6 nM 9.22
CHEMBL4798577 IC50 = 3.0 nM 8.52
CHEMBL4761555 IC50 = 3.0 nM 8.52
CHEMBL4744305 IC50 = 3.0 nM 8.52
CHEMBL107 COLCHICINE IC50 = 3.0 nM 8.52
CHEMBL4789567 IC50 = 16.0 nM 7.80
CHEMBL4763029 IC50 = 16.0 nM 7.80
CHEMBL4786968 IC50 = 16.0 nM 7.80
CHEMBL4794634 IC50 = 16.0 nM 7.80
CHEMBL4741408 IC50 = 16.0 nM 7.80
CHEMBL4759719 IC50 = 80.0 nM 7.10
CHEMBL4740897 IC50 = 80.0 nM 7.10
CHEMBL4759452 IC50 = 400.0 nM 6.40