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Assay Detail

CHEMBL4701077

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BTK in human whole blood assessed as reduction in BTK phosphorylation measured after 30 mins by phosphotyrosine detection method
14
Total Activities
8
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of BIIB068: A Selective, Potent, Reversible Bruton's Tyrosine Kinase Inhibitor as an Orally Efficacious Agent for Autoimmune Diseases.
Ma B,Bohnert T,Otipoby KL,Tien E,Arefayene M,Bai J,Bajrami B,Bame E,Chan TR,Humora M,MacPhee JM,Marcotte D,Mehta D,Metrick CM,Moniz G,Polack E,Poreci U,Prefontaine A,Sheikh S,Schroeder P,Smirnakis K,Zhang L,Zheng F,Hopkins BT
J Med Chem (2020) 63 : 12526 -12541
PubMed 32696648 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.03 6.92
fIC50 6 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4744041 2 6.92
CHEMBL4782313 2 6.46
CHEMBL4754065 2 6.37
CHEMBL4796367 2 6.19
CHEMBL4749757 2 5.85
CHEMBL4746275 1 5.28
CHEMBL4756023 1 5.11
CHEMBL4760128 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4744041 IC50 = 120.0 nM 6.92
CHEMBL4782313 IC50 = 350.0 nM 6.46
CHEMBL4754065 IC50 = 430.0 nM 6.37
CHEMBL4796367 IC50 = 650.0 nM 6.19
CHEMBL4749757 IC50 = 1400.0 nM 5.85
CHEMBL4746275 IC50 = 5300.0 nM 5.28
CHEMBL4756023 IC50 = 7800.0 nM 5.11
CHEMBL4760128 IC50 > 10000.0 nM -
CHEMBL4754065 fIC50 = 1.3 nM -
CHEMBL4749757 fIC50 = 9.8 nM -
CHEMBL4760128 fIC50 = 7.0 nM -
CHEMBL4782313 fIC50 = 0.7 nM -
CHEMBL4796367 fIC50 = 5.9 nM -
CHEMBL4744041 fIC50 = 7.1 nM -