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Assay Detail

CHEMBL4707447

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Biased agonist activity at human recombinant 5-HT1A receptor stably expressed in CHO-K1 cells assessed as increase in ERK1/2 phosphorylation levels incubated for 15 mins by Alphalisa assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 1A (CHEMBL214)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel Aryloxyethyl Derivatives of 1-(1-Benzoylpiperidin-4-yl)methanamine as the Extracellular Regulated Kinases 1/2 (ERK1/2) Phosphorylation-Preferring Serotonin 5-HT Receptor-Biased Agonists with Robust Antidepressant-like Activity.
Sniecikowska J,Gluch-Lutwin M,Bucki A,Więckowska A,Siwek A,Jastrzebska-Wiesek M,Partyka A,Wilczyńska D,Pytka K,Pociecha K,Cios A,Wyska E,Wesołowska A,Pawłowski M,Varney MA,Newman-Tancredi A,Kolaczkowski M
J Med Chem (2019) 62 : 2750 -2771
PubMed 30721053 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 8.02 9.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4753328 1 9.37
CHEMBL4741857 1 9.10
CHEMBL4754941 1 9.06
CHEMBL230963 1 8.33
CHEMBL4753414 1 8.31
CHEMBL56 8-OH-DPAT 1 8.09
CHEMBL4786537 1 7.90
CHEMBL4759629 1 7.89
CHEMBL4754363 1 7.88
CHEMBL49 BUSPIRONE 4.0 1 7.82
CHEMBL4740097 1 7.76
CHEMBL4742122 1 7.62
CHEMBL4789505 1 7.59
CHEMBL39 SEROTONIN 3.0 1 7.48
CHEMBL4746734 1 7.28
CHEMBL4793515 1 6.77

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4753328 EC50 = 0.4266 nM 9.37
CHEMBL4741857 EC50 = 0.7943 nM 9.10
CHEMBL4754941 EC50 = 0.871 nM 9.06
CHEMBL230963 EC50 = 4.677 nM 8.33
CHEMBL4753414 EC50 = 4.898 nM 8.31
CHEMBL56 8-OH-DPAT EC50 = 8.128 nM 8.09
CHEMBL4786537 EC50 = 12.59 nM 7.90
CHEMBL4759629 EC50 = 12.88 nM 7.89
CHEMBL4754363 EC50 = 13.18 nM 7.88
CHEMBL49 BUSPIRONE EC50 = 15.14 nM 7.82
CHEMBL4740097 EC50 = 17.38 nM 7.76
CHEMBL4742122 EC50 = 23.99 nM 7.62
CHEMBL4789505 EC50 = 25.7 nM 7.59
CHEMBL39 SEROTONIN EC50 = 33.11 nM 7.48
CHEMBL4746734 EC50 = 52.48 nM 7.28
CHEMBL4793515 EC50 = 169.82 nM 6.77