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Assay Detail

CHEMBL4708519

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Functional
Antiproliferative activity against human NCI-H1975 cells harboring EGFR T790M/L858R double mutant assessed as inhibition of cell viability measured after 72 hrs by MTT assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type NCI-H1975
Confidence 1 — Organism
Curated By Autocuration

Target

NCI-H1975 (CHEMBL614348)
Type CELL-LINE
Organism Homo sapiens

Publication

Design, synthesis and antitumor activity of novel thiophene-pyrimidine derivatives as EGFR inhibitors overcoming T790M and L858R/T790M mutations.
Xiao Z,Zhou Z,Chu C,Zhang Q,Zhou L,Yang Z,Li X,Yu L,Zheng P,Xu S,Zhu W
Eur J Med Chem (2020) 203 : 112511 -112511
PubMed 32679450 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.38 7.13

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3353410 OSIMERTINIB 4.0 1 7.13
CHEMBL4800491 1 6.68
CHEMBL4787616 1 6.38
CHEMBL3786343 OLMUTINIB 4.0 1 6.28
CHEMBL4789286 1 6.16
CHEMBL4762165 1 5.67
CHEMBL4779676 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3353410 OSIMERTINIB IC50 = 73.4 nM 7.13
CHEMBL4800491 IC50 = 206.6 nM 6.68
CHEMBL4787616 IC50 = 418.0 nM 6.38
CHEMBL3786343 OLMUTINIB IC50 = 520.5 nM 6.28
CHEMBL4789286 IC50 = 699.2 nM 6.16
CHEMBL4762165 IC50 = 2139.0 nM 5.67
CHEMBL4779676 IC50 - -