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Assay Detail

CHEMBL4710183

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant NPP1 expressed in COS-7 cell membrane at 100 uM using p-nitrophenyl thymidine monophosphate substrate incubated for 35 mins by spectrophotometric analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS-7
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Synthesis of biphenyl oxazole derivatives via Suzuki coupling and biological evaluations as nucleotide pyrophosphatase/phosphodiesterase-1 and -3 inhibitors.
Ahmad H,Ullah S,Rahman F,Saeed A,Pelletier J,Sévigny J,Hassan A,Iqbal J
Eur J Med Chem (2020) 208 : 112759 -112759
PubMed 32883636 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.65 6.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4747297 1 6.82
CHEMBL4793019 1 6.38
CHEMBL4758988 1 5.84
CHEMBL4753995 1 5.62
CHEMBL4741253 1 5.59
CHEMBL265502 SURAMIN 3.0 1 5.11
CHEMBL4761471 1 5.04
CHEMBL4759734 1 4.76

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4747297 IC50 = 150.0 nM 6.82
CHEMBL4793019 IC50 = 420.0 nM 6.38
CHEMBL4758988 IC50 = 1440.0 nM 5.84
CHEMBL4753995 IC50 = 2410.0 nM 5.62
CHEMBL4741253 IC50 = 2560.0 nM 5.59
CHEMBL265502 SURAMIN IC50 = 7770.0 nM 5.11
CHEMBL4761471 IC50 = 9230.0 nM 5.04
CHEMBL4759734 IC50 = 17200.0 nM 4.76