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Assay Detail

CHEMBL4710397

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BRD2 BD2 (unknown origin) preincubated for 15 mins followed by peptide addition and measured after 60 mins by TR-FRET assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 2 (CHEMBL1293289)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and biological evaluation of indole-2-one derivatives as potent BRD4 inhibitors.
Xu Y,Zhang XJ,Li WB,Wang XR,Wang S,Qiao XP,Chen SW
Eur J Med Chem (2020) 208 : 112780 -112780
PubMed 32883643 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.80 8.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1957266 1 8.17
CHEMBL4752543 1 7.75
CHEMBL4743540 1 7.66
CHEMBL4798353 1 7.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1957266 IC50 = 6.7 nM 8.17
CHEMBL4752543 IC50 = 18.0 nM 7.75
CHEMBL4743540 IC50 = 22.0 nM 7.66
CHEMBL4798353 IC50 = 25.0 nM 7.60