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Assay Detail

CHEMBL4712181

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at recombinant human N-terminal epitope tagged/eYFP-fused FLAG-tagged GPR84 expressed in Flp-In TREx 293 cells assessed as inhibition of DIM-induced [35S]GTPgammaS binding pre-incubated for 15 mins followed by [35S]GTPgammaS addition and measured after 45 mins by liquid scintillation spectrometry
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Flp-In-T-REx-293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled receptor 84 (CHEMBL3714079)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Modulation of the G-Protein-Coupled Receptor 84 (GPR84) by Agonists and Antagonists.
Chen LH,Zhang Q,Xie X,Nan FJ
J Med Chem (2020) 63 : 15399 -15409
PubMed 33267584 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 7.34 7.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3715656 1 7.68
CHEMBL3715918 1 7.26
CHEMBL3717743 1 7.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3715656 IC50 = 21.0 nM 7.68
CHEMBL3715918 IC50 = 55.0 nM 7.26
CHEMBL3717743 IC50 = 86.0 nM 7.07