Assay Detail
Functional
CHEMBL4712184
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antagonist activity at mouse Galphai2-coupled GPR84 expressed in human Flp-In-T-REx-293 cell membrane assessed as reduction in ZQ-16-induced stimulation of [35S]GTPgammaS binding preincubated for 15 mins followed by ZQ-16 addition and subsequent addition of [35S]GTPgammaS measured after 60 mins by liquid scintillation spectroscopy
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Mus musculus |
| Cell Type | Flp-In-T-REx-293 |
| Subcellular | Cell membrane |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Modulation of the G-Protein-Coupled Receptor 84 (GPR84) by Agonists and Antagonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 6.90 | 6.90 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3715656 | — | — | 1 | 6.90 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3715656 | — | IC50 | = | 126.0 | nM | 6.90 |