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Assay Detail

CHEMBL4714732

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full-length His-tagged TAK1-TAB1 fusion protein (437 to 504 residues) expressed in baculovirus expression system using fluorescein-MAP2K1 as substrate preincubated for 30 mins followed by substrate addition and measured after 60 mins in presence of ATP by lanthascreen TR-FRET assay
44
Total Activities
44
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

TAK1/TAB1 (CHEMBL3038499)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of 2,4-1H-Imidazole Carboxamides as Potent and Selective TAK1 Inhibitors.
Veerman JJN,Bruseker YB,Damen E,Heijne EH,van Bruggen W,Hekking KFW,Winkel R,Hupp CD,Keefe AD,Liu J,Thomson HA,Zhang Y,Cuozzo JW,McRiner AJ,Mulvihill MJ,van Rijnsbergen P,Zech B,Renzetti LM,Babiss L,Müller G
ACS Med Chem Lett (2021) 12 : 555 -562
PubMed 33859795 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 44 6.05 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4796239 1 8.70
CHEMBL4752978 1 8.30
CHEMBL2408611 1 8.00
CHEMBL3823738 1 7.52
CHEMBL388978 STAUROSPORINE 1 7.41
CHEMBL4795442 1 6.52
CHEMBL4786426 1 6.52
CHEMBL4799816 1 6.30
CHEMBL4743586 1 6.05
CHEMBL4793594 1 6.05
CHEMBL4753680 1 6.00
CHEMBL4784347 1 5.92
CHEMBL4754286 1 5.92
CHEMBL4787765 1 5.89
CHEMBL4748960 1 5.77
CHEMBL4742800 1 5.75
CHEMBL4787363 1 5.75
CHEMBL4764405 1 5.64
CHEMBL4760000 1 5.58
CHEMBL4790917 1 5.58
CHEMBL4779445 1 5.55
CHEMBL4742896 1 5.54
CHEMBL4758712 1 5.50
CHEMBL4740886 1 5.47
CHEMBL4776433 1 5.47
CHEMBL4785659 1 5.46
CHEMBL4760703 1 5.35
CHEMBL4785897 1 5.24
CHEMBL4758714 1 4.58
CHEMBL4750042 1 4.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4796239 IC50 = 2.0 nM 8.70
CHEMBL4752978 IC50 = 5.0 nM 8.30
CHEMBL2408611 IC50 = 10.0 nM 8.00
CHEMBL3823738 IC50 = 30.0 nM 7.52
CHEMBL388978 STAUROSPORINE IC50 = 39.0 nM 7.41
CHEMBL4795442 IC50 = 300.0 nM 6.52
CHEMBL4786426 IC50 = 300.0 nM 6.52
CHEMBL4799816 IC50 = 500.0 nM 6.30
CHEMBL4743586 IC50 = 900.0 nM 6.05
CHEMBL4793594 IC50 = 900.0 nM 6.05
CHEMBL4753680 IC50 = 1000.0 nM 6.00
CHEMBL4754286 IC50 = 1200.0 nM 5.92
CHEMBL4784347 IC50 = 1200.0 nM 5.92
CHEMBL4787765 IC50 = 1300.0 nM 5.89
CHEMBL4748960 IC50 = 1700.0 nM 5.77
CHEMBL4742800 IC50 = 1800.0 nM 5.75
CHEMBL4787363 IC50 = 1800.0 nM 5.75
CHEMBL4764405 IC50 = 2300.0 nM 5.64
CHEMBL4760000 IC50 = 2600.0 nM 5.58
CHEMBL4790917 IC50 = 2600.0 nM 5.58
CHEMBL4779445 IC50 = 2800.0 nM 5.55
CHEMBL4742896 IC50 = 2900.0 nM 5.54
CHEMBL4758712 IC50 = 3200.0 nM 5.50
CHEMBL4776433 IC50 = 3400.0 nM 5.47
CHEMBL4740886 IC50 = 3400.0 nM 5.47
CHEMBL4785659 IC50 = 3500.0 nM 5.46
CHEMBL4760703 IC50 = 4500.0 nM 5.35
CHEMBL4785897 IC50 = 5700.0 nM 5.24
CHEMBL4758714 IC50 = 26000.0 nM 4.58
CHEMBL4750042 IC50 = 59000.0 nM 4.23
CHEMBL4792942 IC50 > 100000.0 nM -
CHEMBL4746806 IC50 > 10000.0 nM -
CHEMBL4763165 IC50 > 100000.0 nM -
CHEMBL4777281 IC50 > 10000.0 nM -
CHEMBL4751348 IC50 > 10000.0 nM -
CHEMBL4763783 IC50 > 10000.0 nM -
CHEMBL4778091 IC50 > 10000.0 nM -
CHEMBL4763081 IC50 > 10000.0 nM -
CHEMBL4762449 IC50 > 10000.0 nM -
CHEMBL4746405 IC50 > 10000.0 nM -
CHEMBL4789883 IC50 > 10000.0 nM -
CHEMBL4781976 IC50 > 10000.0 nM -
CHEMBL4785563 IC50 > 10000.0 nM -
CHEMBL4741059 IC50 > 10000.0 nM -