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Assay Detail

CHEMBL4718608

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antioxidant activity in human SH-SY5Y cells assessed as inhibition of H2O2-induced DCFH oxidation measured after 1 hr by DCFH-DA dye based fluorescence assay
32
Total Activities
16
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type SH-SY5Y
Confidence 1 — Organism
Curated By Autocuration

Target

SH-SY5Y (CHEMBL614910)
Type CELL-LINE
Organism Homo sapiens

Publication

Synthesis and in vitro, ex-vivo and in vivo activity of hybrid compounds linking a potent ROS and RNS scavenger activity with diverse substrates addressed to pass across the blood-brain barrier.
Vázquez-Jiménez L,Garrido M,Miceli M,Prats E,Ferrer-Montiel A,Teixidó M,Jimeno C,Messeguer A
Eur J Med Chem (2016) 123 : 788 -802
PubMed 27541262 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Activity 16 - -
IC50 16 4.33 4.67

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4747699 2 4.67
CHEMBL4755403 2 4.66
CHEMBL4747354 2 4.49
CHEMBL4793012 2 4.45
CHEMBL4740326 2 4.38
CHEMBL4744769 2 4.36
CHEMBL4793596 2 4.35
CHEMBL4760848 2 4.30
CHEMBL4793823 2 4.30
CHEMBL4799132 2 4.15
CHEMBL4797546 2 4.11
CHEMBL153 TROLOX 2 4.08
CHEMBL4763677 2 4.03
CHEMBL4744943 2 -
CHEMBL4790338 2 -
CHEMBL4756734 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4747699 IC50 = 21600.0 nM 4.67
CHEMBL4755403 IC50 = 21700.0 nM 4.66
CHEMBL4747354 IC50 = 32300.0 nM 4.49
CHEMBL4793012 IC50 = 35600.0 nM 4.45
CHEMBL4740326 IC50 = 41400.0 nM 4.38
CHEMBL4744769 IC50 = 43800.0 nM 4.36
CHEMBL4793596 IC50 = 44500.0 nM 4.35
CHEMBL4793823 IC50 = 50000.0 nM 4.30
CHEMBL4760848 IC50 = 50300.0 nM 4.30
CHEMBL4799132 IC50 = 71200.0 nM 4.15
CHEMBL4797546 IC50 = 78000.0 nM 4.11
CHEMBL153 TROLOX IC50 = 82300.0 nM 4.08
CHEMBL4763677 IC50 = 92600.0 nM 4.03
CHEMBL4747699 Activity - -
CHEMBL4747354 Activity - -
CHEMBL4755403 Activity - -
CHEMBL153 TROLOX Activity - -
CHEMBL4744943 IC50 > 200000.0 nM -
CHEMBL4763677 Activity - -
CHEMBL4744769 Activity - -
CHEMBL4790338 IC50 > 300000.0 nM -
CHEMBL4740326 Activity - -
CHEMBL4793823 Activity - -
CHEMBL4756734 IC50 > 200000.0 nM -
CHEMBL4756734 Activity - -
CHEMBL4799132 Activity - -
CHEMBL4797546 Activity - -
CHEMBL4790338 Activity - -
CHEMBL4793012 Activity - -
CHEMBL4760848 Activity - -
CHEMBL4744943 Activity - -
CHEMBL4793596 Activity - -