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Assay Detail

CHEMBL4719151

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal His-tagged Pin1 expressed in Escherichia coli BL21 using Suc-Ala-Glu-Pro-Phe-4-nitroanilide as substrate incubated for 10 mins followed by substrate addition and measured for 90 sec by spectrophotometry
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (CHEMBL2288)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and Pin1 inhibitory activity of thiazole derivatives.
Zhao H,Cui G,Jin J,Chen X,Xu B
Bioorg Med Chem (2016) 24 : 5911 -5920
PubMed 27692510 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 5.24 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL585917 1 7.30
CHEMBL3322225 1 5.53
CHEMBL4792060 1 5.53
CHEMBL4757254 1 5.50
CHEMBL4744323 1 5.49
CHEMBL4779755 1 5.34
CHEMBL4781756 1 5.33
CHEMBL4791317 1 5.33
CHEMBL4777545 1 5.30
CHEMBL4745481 1 5.29
CHEMBL4745229 1 5.27
CHEMBL4764554 1 5.27
CHEMBL4740631 1 5.26
CHEMBL4759628 1 5.22
CHEMBL4762421 1 5.09
CHEMBL4782310 1 5.03
CHEMBL4761085 1 5.03
CHEMBL4795100 1 4.96
CHEMBL4747974 1 4.87
CHEMBL4796536 1 4.85
CHEMBL4740637 1 4.83
CHEMBL4791517 1 4.74
CHEMBL4745826 1 4.68
CHEMBL4797808 1 4.63

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL585917 IC50 = 50.0 nM 7.30
CHEMBL3322225 IC50 = 2960.0 nM 5.53
CHEMBL4792060 IC50 = 2930.0 nM 5.53
CHEMBL4757254 IC50 = 3150.0 nM 5.50
CHEMBL4744323 IC50 = 3220.0 nM 5.49
CHEMBL4779755 IC50 = 4570.0 nM 5.34
CHEMBL4781756 IC50 = 4680.0 nM 5.33
CHEMBL4791317 IC50 = 4670.0 nM 5.33
CHEMBL4777545 IC50 = 5020.0 nM 5.30
CHEMBL4745481 IC50 = 5180.0 nM 5.29
CHEMBL4745229 IC50 = 5420.0 nM 5.27
CHEMBL4764554 IC50 = 5380.0 nM 5.27
CHEMBL4740631 IC50 = 5500.0 nM 5.26
CHEMBL4759628 IC50 = 6040.0 nM 5.22
CHEMBL4762421 IC50 = 8110.0 nM 5.09
CHEMBL4782310 IC50 = 9350.0 nM 5.03
CHEMBL4761085 IC50 = 9440.0 nM 5.03
CHEMBL4795100 IC50 = 11100.0 nM 4.96
CHEMBL4747974 IC50 = 13400.0 nM 4.87
CHEMBL4796536 IC50 = 14000.0 nM 4.85
CHEMBL4740637 IC50 = 14800.0 nM 4.83
CHEMBL4791517 IC50 = 18100.0 nM 4.74
CHEMBL4745826 IC50 = 21100.0 nM 4.68
CHEMBL4797808 IC50 = 23400.0 nM 4.63