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Assay Detail

CHEMBL4719942

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human recombinant mGlu3 receptor stably expressed in golden hamster AV12 cells co-expressing rat EAAT1 assessed as reduction in DCG IV-induced inhibition of forskolin-stimulated cAMP accumulation preincubated for 20 mins followed by incubation with cAMP-d2 conjugate and anti-cAMP cryptate for 1 hr by HTRF assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type AV12
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 3 (CHEMBL2888)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of (1S,2R,3S,4S,5R,6R)-2-Amino-3-[(3,4-difluorophenyl)sulfanylmethyl]-4-hydroxy-bicyclo[3.1.0]hexane-2,6-dicarboxylic Acid Hydrochloride (LY3020371·HCl): A Potent, Metabotropic Glutamate 2/3 Receptor Antagonist with Antidepressant-Like Activity.
Chappell MD,Li R,Smith SC,Dressman BA,Tromiczak EG,Tripp AE,Blanco MJ,Vetman T,Quimby SJ,Matt J,Britton TC,Fivush AM,Schkeryantz JM,Mayhugh D,Erickson JA,Bures MG,Jaramillo C,Carpintero M,Diego JE,Barberis M,Garcia-Cerrada S,Soriano JF,Antonysamy S,Atwell S,MacEwan I,Condon B,Sougias C,Wang J,Zhang A,Conners K,Groshong C,Wasserman SR,Koss JW,Witkin JM,Li X,Overshiner C,Wafford KA,Seidel W,Wang XS,Heinz BA,Swanson S,Catlow JT,Bedwell DW,Monn JA,Mitch CH,Ornstein PL
J Med Chem (2016) 59 : 10974 -10993
PubMed 28002967 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.92 8.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4751065 1 8.21
CHEMBL432038 1 8.11
CHEMBL4746125 1 7.91
CHEMBL4759992 1 7.79
CHEMBL4780402 1 7.57
CHEMBL4776989 1 7.38
CHEMBL4748699 1 7.25
CHEMBL4760856 1 7.21
CHEMBL4757159 1 7.21
CHEMBL3956934 1 6.96
CHEMBL4756556 1 6.79
CHEMBL4759209 1 6.70
CHEMBL4784701 1 6.57
CHEMBL4781638 1 6.26
CHEMBL4755204 1 6.01
CHEMBL4779554 1 5.67
CHEMBL4778355 1 5.62
CHEMBL4749728 1 5.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4751065 IC50 = 6.2 nM 8.21
CHEMBL432038 IC50 = 7.7 nM 8.11
CHEMBL4746125 IC50 = 12.4 nM 7.91
CHEMBL4759992 IC50 = 16.1 nM 7.79
CHEMBL4780402 IC50 = 27.0 nM 7.57
CHEMBL4776989 IC50 = 41.9 nM 7.38
CHEMBL4748699 IC50 = 56.5 nM 7.25
CHEMBL4760856 IC50 = 62.3 nM 7.21
CHEMBL4757159 IC50 = 61.1 nM 7.21
CHEMBL3956934 IC50 = 110.0 nM 6.96
CHEMBL4756556 IC50 = 163.0 nM 6.79
CHEMBL4759209 IC50 = 202.0 nM 6.70
CHEMBL4784701 IC50 = 272.0 nM 6.57
CHEMBL4781638 IC50 = 547.0 nM 6.26
CHEMBL4755204 IC50 = 967.0 nM 6.01
CHEMBL4779554 IC50 = 2160.0 nM 5.67
CHEMBL4778355 IC50 = 2410.0 nM 5.62
CHEMBL4749728 IC50 = 4560.0 nM 5.34