Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4721175

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal GST tagged EGFR L858R mutant (669 to 1210 residues) expressed in insect expression system using peptide as substrate incubated for 2 hrs followed by substrate addition and measured after 30 mins by TR-FRET assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel quinazoline derivatives bearing various 6-benzamide moieties as highly selective and potent EGFR inhibitors.
Hou W,Ren Y,Zhang Z,Sun H,Ma Y,Yan B
Bioorg Med Chem (2018) 26 : 1740 -1750
PubMed 29523467 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 7.69 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL31965 CANERTINIB 3.0 1 10.00
CHEMBL1173655 AFATINIB 4.0 1 9.70
CHEMBL3353410 OSIMERTINIB 4.0 1 9.30
CHEMBL1229592 1 9.10
CHEMBL4792426 1 8.72
CHEMBL4750196 1 8.57
CHEMBL4777353 1 8.42
CHEMBL4745521 1 8.42
CHEMBL4757724 1 8.42
CHEMBL4753193 1 7.92
CHEMBL4793852 1 7.85
CHEMBL4790583 1 7.77
CHEMBL4796407 1 7.75
CHEMBL4763967 1 7.50
CHEMBL4743173 1 7.13
CHEMBL4747893 1 6.71
CHEMBL4755444 1 6.57
CHEMBL4763697 1 6.37
CHEMBL4790485 1 6.33
CHEMBL4757531 1 6.32
CHEMBL4756049 1 6.18
CHEMBL4777554 1 6.13
CHEMBL4784185 1 5.65
CHEMBL4749765 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL31965 CANERTINIB IC50 = 0.1 nM 10.00
CHEMBL1173655 AFATINIB IC50 = 0.2 nM 9.70
CHEMBL3353410 OSIMERTINIB IC50 = 0.5 nM 9.30
CHEMBL1229592 IC50 = 0.8 nM 9.10
CHEMBL4792426 IC50 = 1.9 nM 8.72
CHEMBL4750196 IC50 = 2.7 nM 8.57
CHEMBL4777353 IC50 = 3.8 nM 8.42
CHEMBL4745521 IC50 = 3.8 nM 8.42
CHEMBL4757724 IC50 = 3.8 nM 8.42
CHEMBL4753193 IC50 = 12.0 nM 7.92
CHEMBL4793852 IC50 = 14.0 nM 7.85
CHEMBL4790583 IC50 = 17.0 nM 7.77
CHEMBL4796407 IC50 = 18.0 nM 7.75
CHEMBL4763967 IC50 = 32.0 nM 7.50
CHEMBL4743173 IC50 = 74.0 nM 7.13
CHEMBL4747893 IC50 = 194.0 nM 6.71
CHEMBL4755444 IC50 = 270.0 nM 6.57
CHEMBL4763697 IC50 = 427.0 nM 6.37
CHEMBL4790485 IC50 = 463.0 nM 6.33
CHEMBL4757531 IC50 = 475.0 nM 6.32
CHEMBL4756049 IC50 = 666.0 nM 6.18
CHEMBL4777554 IC50 = 735.0 nM 6.13
CHEMBL4784185 IC50 = 2245.0 nM 5.65
CHEMBL4749765 IC50 > 200.0 nM -