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Assay Detail

CHEMBL4721180

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal GST tagged EGFR (669 to 1210 residues) expressed in insect expression system using peptide as substrate incubated for 2 hrs followed by substrate addition and measured after 30 mins by TR-FRET assay
36
Total Activities
36
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel quinazoline derivatives bearing various 6-benzamide moieties as highly selective and potent EGFR inhibitors.
Hou W,Ren Y,Zhang Z,Sun H,Ma Y,Yan B
Bioorg Med Chem (2018) 26 : 1740 -1750
PubMed 29523467 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 36 7.49 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL939 GEFITINIB 4.0 1 9.30
CHEMBL4789811 1 8.89
CHEMBL4792426 1 8.59
CHEMBL4790655 1 8.52
CHEMBL4752951 1 8.37
CHEMBL4750196 1 8.30
CHEMBL4777353 1 8.20
CHEMBL4757724 1 8.18
CHEMBL4782025 1 8.08
CHEMBL4745521 1 8.04
CHEMBL4754811 1 7.89
CHEMBL4753193 1 7.89
CHEMBL4743566 1 7.85
CHEMBL4793852 1 7.68
CHEMBL4776057 1 7.62
CHEMBL4755832 1 7.57
CHEMBL4749765 1 7.55
CHEMBL4741678 1 7.54
CHEMBL4760864 1 7.52
CHEMBL4747893 1 7.41
CHEMBL4750608 1 7.37
CHEMBL4790583 1 7.36
CHEMBL4796407 1 7.30
CHEMBL4759414 1 7.29
CHEMBL4763967 1 7.28
CHEMBL4760606 1 7.24
CHEMBL4743173 1 7.17
CHEMBL4757531 1 6.77
CHEMBL4777554 1 6.76
CHEMBL4751768 1 6.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL939 GEFITINIB IC50 = 0.5 nM 9.30
CHEMBL4789811 IC50 = 1.3 nM 8.89
CHEMBL4792426 IC50 = 2.6 nM 8.59
CHEMBL4790655 IC50 = 3.0 nM 8.52
CHEMBL4752951 IC50 = 4.3 nM 8.37
CHEMBL4750196 IC50 = 5.0 nM 8.30
CHEMBL4777353 IC50 = 6.3 nM 8.20
CHEMBL4757724 IC50 = 6.6 nM 8.18
CHEMBL4782025 IC50 = 8.3 nM 8.08
CHEMBL4745521 IC50 = 9.2 nM 8.04
CHEMBL4754811 IC50 = 13.0 nM 7.89
CHEMBL4753193 IC50 = 13.0 nM 7.89
CHEMBL4743566 IC50 = 14.0 nM 7.85
CHEMBL4793852 IC50 = 21.0 nM 7.68
CHEMBL4776057 IC50 = 24.0 nM 7.62
CHEMBL4755832 IC50 = 27.0 nM 7.57
CHEMBL4749765 IC50 = 28.0 nM 7.55
CHEMBL4741678 IC50 = 29.0 nM 7.54
CHEMBL4760864 IC50 = 30.0 nM 7.52
CHEMBL4747893 IC50 = 39.0 nM 7.41
CHEMBL4750608 IC50 = 43.0 nM 7.37
CHEMBL4790583 IC50 = 44.0 nM 7.36
CHEMBL4796407 IC50 = 50.0 nM 7.30
CHEMBL4759414 IC50 = 50.9 nM 7.29
CHEMBL4763967 IC50 = 53.0 nM 7.28
CHEMBL4760606 IC50 = 57.0 nM 7.24
CHEMBL4743173 IC50 = 68.0 nM 7.17
CHEMBL4757531 IC50 = 169.0 nM 6.77
CHEMBL4777554 IC50 = 172.0 nM 6.76
CHEMBL4751768 IC50 = 242.4 nM 6.62
CHEMBL4762998 IC50 = 365.0 nM 6.44
CHEMBL4755444 IC50 = 374.0 nM 6.43
CHEMBL4756049 IC50 = 390.0 nM 6.41
CHEMBL4790485 IC50 = 503.0 nM 6.30
CHEMBL4763697 IC50 = 722.0 nM 6.14
CHEMBL4784185 IC50 = 2426.0 nM 5.62